You have cloned the gene for a human erythrocyte protein, which you suspect is a membrane protein. You deduce the amino acid sequence of the protein from the nucleotide sequence of the gene. From the amino acid sequence alone, you evaluate the possibility that the protein is an integral protein. How could you determine whether the protein is an integral protein? Use a hydropathy plot. If the plot shows a hydrophobic region of 20 or more residues, it is likely an integral protein. Use a hydropathy plot. If the plot shows a hydrophilic region of 20 or more residues, it is likely an integral protein. Determine the distribution of proteins. If its distribution is asymmetric, it is likely an integral protein. Determine the distribution of proteins. If its distribution is symmetric, it is likely an integral protein. Suppose the protein proves to be an integral protein with one transmembrane segment. How could you determine whether the protein is oriented with the amino terminus on the outside or the inside of the cell? Treat protein reacts, the amino terminus is with a membrane-impermeant reagent known to react with primary amines. If the of the erythrocyte membrane.
You have cloned the gene for a human erythrocyte protein, which you suspect is a membrane protein. You deduce the amino acid sequence of the protein from the nucleotide sequence of the gene. From the amino acid sequence alone, you evaluate the possibility that the protein is an integral protein. How could you determine whether the protein is an integral protein? Use a hydropathy plot. If the plot shows a hydrophobic region of 20 or more residues, it is likely an integral protein. Use a hydropathy plot. If the plot shows a hydrophilic region of 20 or more residues, it is likely an integral protein. Determine the distribution of proteins. If its distribution is asymmetric, it is likely an integral protein. Determine the distribution of proteins. If its distribution is symmetric, it is likely an integral protein. Suppose the protein proves to be an integral protein with one transmembrane segment. How could you determine whether the protein is oriented with the amino terminus on the outside or the inside of the cell? Treat protein reacts, the amino terminus is with a membrane-impermeant reagent known to react with primary amines. If the of the erythrocyte membrane.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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Transcribed Image Text:You have cloned the gene for a human erythrocyte protein, which you suspect is a membrane protein. You deduce the amino
acid sequence of the protein from the nucleotide sequence of the gene. From the amino acid sequence alone, you evaluate the
possibility that the protein is an integral protein.
How could you determine whether the protein is an integral protein?
Use a hydropathy plot. If the plot shows a hydrophobic region of 20 or more residues, it is likely an integral protein.
Use a hydropathy plot. If the plot shows a hydrophilic region of 20 or more residues, it is likely an integral protein.
Determine the distribution of proteins. If its distribution is asymmetric, it is likely an integral protein.
Determine the distribution of proteins. If its distribution is symmetric, it is likely an integral protein.
Suppose the protein proves to be an integral protein with one transmembrane segment. How could you determine whether the
protein is oriented with the amino terminus on the outside or the inside of the cell?
Treat
protein reacts, the amino terminus is
with a membrane-impermeant reagent known to react with primary amines. If the
of the erythrocyte membrane.
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