You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar, you will be preparing the desired concentration from a prepared stock solution. Complete the information in the table and show your computation. Volume of Volume of diluent Desired Stock Solution stock sol'n needed to make Concentration needed 1000mL media 25% 12.5% 5% 1.5%
Q: Based on the information given, what separation technique(s) is/are most likely feasible? Please…
A: The selective boiling and subsequent condensation of a part in a liquid mixture is referred to as…
Q: You are asked to prepare 20 mL of a 50 mg/mL drug dilution from a stog of 2 g/5 mL. How many…
A: Here, We need to calculate the milliliter of dilutant needed, for that purpose stock solution…
Q: McConkey Agar has been requested by a hospital that is investigating cases of suspected Salmonella…
A: Mcconkey agar is a media for gram negative bacteria.
Q: Louie performed paper chromatography to identify which among the four available reference mixtures -…
A: The technique of separating substances based on the differential migration across sheets of paper is…
Q: In a neutral solution, the [H] is : O 1.0x10-14M zero 1.0×107M 1.0x10-7M
A: The acidity of any solution is determined by the molarity of the Hydrogen ions. So, the pH of any…
Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…
A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…
Q: Why are we using this materials and centrifuging at 15000 rpm? Lipid peroxidation experiment:…
A: The oxidative degeneration of lipids refers to lipid peroxidation, where the electrons from the…
Q: How much 10% dextrose solution and 20% dextrose solution should be mixed to prepare 1 l 12.5%…
A: Dextrose is an intravenous solution made up of a mixture of water and dextrose and used in low blood…
Q: You purchase a premix flour containing iron (Fe), riboflavin, niacin, thiamin, and folate. For…
A: Atomic absorption spectrometry (AAS) is a simple, high-throughput, low-cost technique for analyzing…
Q: Kael needs to prepare Luria Bertani Agar for 40 plates, 15 slants (big tubes), and 15 stabs (small…
A: LBA agar is used for cultivation of recombinant E.coli. it is a type of rich medium it is also used…
Q: Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite…
A: Luminol is a chemical used in many laboratory experiments, like in biotech research lab, forensic…
Q: Calculate the dimensionless spray flux for the normal case and the atypical batch, and explain why…
A: As given in the question, the value of spray flux for: Normal value - 0.52 Atypical value - 1.46…
Q: The BSA stock solution from the previous problem was then diluted to generate a set of standard…
A: CONCENTRATION OF BSA (mg/ml) ABSORBANCE 0 0 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8…
Q: What is displacement value, why is it important in pharmacy calculation? In which pharmaceutical…
A: Pharmacy is the branch of health science that connects medical science with chemistry. It is…
Q: All recipes are given per litre unless specified. Lambda Broth (LB) Plates…
A: Give, The final volume (V2) = 5mL. The stock concentration of MgSO4 (C1)= 1M The desired…
Q: Given the following common laboratory materials, which of the three methods of heat sterilization do…
A: Three Methods of heat sterilisation include: Burning over flame Wet heat (autoclave) Dry heat…
Q: What nutrients do the following media components contain? Peptone Yeast extract Beef…
A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: Calculate the volume of BSA stock that will be required to make the standard solutions needed to…
A: A stock solution is a concentrated solution that is diluted to make a working solution. It is used…
Q: In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the…
A: Kool aid chromatography The blue dye, is more non-polar than the red dye and is attracted more…
Q: If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____.…
A: According to the question, If 10 µL of cells in suspension was mixed with 20 µL of a dye called…
Q: can you show how to calculate the amount of two components for the required volume of a buffer with…
A: Given Values: Concentration of Tris = 0.25 M Concentration of Glycine = 1.92 M Concentration of SDS…
Q: Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction…
A: Sterilization is a physical process that, in microbiology science, plays a vital role in preventing…
Q: Before using the top agar, maltose and MgSO4 must be added. Why are maltose and MgSO4 added after…
A: Given, The stock concentration of maltose is 20% Required concentration is ~0.2% Final volume is…
Q: how to prepare a 100 mL of 4x sds buffer from a 1.0 L of 50x SDS stock solution. Show complete…
A: In order to characterize the cells genetically, it is necessary to isolate the DNA that is present…
Q: Take a look at this graph. The reactant curve is plotted in red, while the product curve is plotted…
A: Quantitative estimation of a substance is a process of determining the amount of substance present…
Q: What is the fluid thioglycollate medium? Please explain the testing and its results and the…
A: Microbiology laboratory uses various kinds of culture media to identify the biochemical…
Q: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in…
A: 1*Erlenmeyer flasks are used to contain liquids and for mixing and heating and cooling, incubation…
Q: The data from thin layer chromatographic separation of analgesics showed ibuprofen distance to be…
A: Ibuprofen is solute here Distance travelled by Ibuprofen = 0.752 mm Distance travelled by solvent =…
Q: Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the…
A: By the gel filtration chromatography the protein or other biological molecules of solutions…
Q: You have a stock solution of 40% hydrogen peroxide. You need to make several dilutions for a…
A: Dilution of Hydrogen peroxide (H2O2): Dilution of Hydrogen peroxide (H2O2), is carried out by adding…
Q: draw a schematic diagram on the Extraction of Flavonoids using the procedures stated below.
A: Extraction of flavonoids
Q: The stabilized ether contains a stadilizer-antioxidant: A) Methyl alcohol in a concentration of not…
A: Asked : Stabilized ether contains a stabilizer-antioxidant from the given options.
Q: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test…
A: In order for the microorganisms to survive and grow under laboratory conditions we need to provide…
Q: You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an…
A: Agarose gel electrophoresis is a technique to study the features of genetic material and proteins.…
Q: What is the chemistry principle behind when (treatment: heavy cream) is subjected to room…
A: Heavy cream temperature is important for the physical characteristics of the cream. At room…
Q: In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials…
A: Sterilization is a process of killing unwanted microbes on the surface of medical or industrial…
Q: For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h,…
A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…
Q: You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table.…
A: We are having the concentration of the ammonium nitrate and potassium nitrate at 1 litre or 1000 ml…
Q: 6- 2- 1- 45 90 135 180 225 270 315 360 405 450 495 540 58! [Substrate] (nM) rate (nM/sec) is
A:
Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…
A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…
Q: What is the chemistry principle behind when (treatment: heavy cream) is subjected to CHILLED…
A: Here the experiment is basically the chilling effect on heavy cream and studing the pattern of its…
Q: a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product…
A: Given values in the question are as follows: mAb = 120 mgl Total volume of the solution = 5 l…
Q: Calculate the amount of each solution needed to make 750 mL if a dextrose 25% solution given only a…
A: Given- We make 25% dextrose of 750ml by using 60% and 5% dextrose. Use alligation method for such…
Q: It was suspected that a particular mixture contained three components: X, Y and Z. To check this,…
A: Thin-layer chromatography is a technique for separating non-volatile mixes from volatile mixtures.…
Q: You want to use a serial dilution to make a 1/50 dilution. The first dilution you make is a 1/5…
A: Introduction Dilution is a process by which the concentrations of solute are lowered down in a…
Q: An unknown sample was titrated with CH3COOH. After making the titration curve, no steep change was…
A: CH3COOH is a weak acid hence it will not dissociate completely in the aqueous solution to form…
Q: A biochemist carefully measures the molarity of salt in 56. mL of photobacterium cell growth medium…
A: GIVEN: The initial volume of the photobacterium cell growth medium (V1)= 56 mL Concentration of the…
Q: Three sugars (Sugar A, Band C) were applied to a line as part of the set-up of a Paper…
A: Paper chromatography is the basic technique to separate dissolved chemical substances from the…
Q: True or false: Ethidium bromide is added to your hot liquid agar solution to help sepa bands by size…
A: Agarose gel electrophoresis is one of the major techniques that is used in molecular biology…
Q: Suppose you have an initial chlorine concentration of 5%. Then out of that, you are told to prepare…
A: The concentration of a solution: The concentration of a solution is defined as the amount of the…
Step by step
Solved in 4 steps with 1 images
- Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non- fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare 300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your calculations. *A sample calculation was made for you. Peptone = 300 ml x 0.5% = 1.5 g Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019) Ingredients In % In Grams/300ml Peptone 0.5 *1.5 Meat extract 0.3 Agar 1.5 Distilled Water As neededYou are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table. Answer the following question: 1. Prepare 250 mL stock solution (200X more concentrated) of ammonium nitrate and KNO3. 2. From the prepared ammonium nitrate stock solution above, calculate the volume required to prepare 200 mL MS medium.Answer the ff and state references: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria What is the total volume of the medium needed? What is the amount of each component needed, given the following media composition per liter of distilled water: Component g/L tryptone 10.0 NaCl 5.0 yeast extract 10.0 agar 15.0
- On a paper, draw a schematic diagram on the Extraction of Flavonoids using the procedures stated below. Procedure: Extraction of Flavonoids Weigh 10 g of the shade dried plant sample and chop into small pieces. Macerate with 200 mL 70% ethanol for 3 days at room temperature (25°C). Filter the extracts in vacuum using Whatman filter. Evaporate to dryness. Weigh the residue and compute the percentage yield.Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.
- You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
- All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…Compute the cfu/g (lactic acid bacteria and coliforms) of burong mustasa samples given the following values below. Show your solution. 1st Sampling 2nd Sampling Media 10-2 10-3 10-4 10-2 10-3 10-4 TNTC. 252, 248, VRBA 28, 22, 31 26, 33 0, 0 0, 0 TNTC 232 0, 0 TNTC, TNTC TNTC, TNTC MRS 38, 42 0,0 152, 132 Volume plated: 0.1mlGiven: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the distance of one ocular division? Show your solution.