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Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn
and coconut water 10%.
Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn
5gm/100ml. If you need anything else then
you add accordingly. BAP has a MW of
225.3. Sucrose and Myo-inositol is not mentioned in the question. These you
need to add. Consistency of the medium is also mentioned.
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- Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.
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- The number of cells in a culture is estimated based on turbidity. If the following standards are used for comparison: Number of cells/mL Optical Density (measure of turbidity) 0.025 0.050 000'000'৮ 000 000'000' కరరక How many cells are present in a culture that has an optical density of 0.075? O A. Less than 1,000,000 O B. Between 2,000,000 and 4,000,000 O C. 16,000,000 O D. Between 4,000,000 and 8,000,000 Reset SelectionChoose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible6. You were asked by your laboratory instructor to prepare the following: 12 plates (use maximum volume) and 6 big slants of Malt Yeast Extract Glucose Agar. Calculate for the total volume of the media you need to prepare, and the amount (in grams) needed to be weighed for each component. Refer to the table below. Note: Show all computations. Use 3 significant figures for your final answer. Amount (per 200 ml) 0.6 g 0.6 g 4.0 g 3.4 g Malt Yeast Extract Glucose Agar Malt extract Yeast extract Glucose Peptone Agar 1.7 % Table 1. Glassware used in media preparation and their approximate volume capacity. Glassware Approximate volume* Petri dish 15-20 ml Big test tubes (18 mm diameter) as stabs/broth 10 ml as slants 6-8 ml Small tubes (13 mm diameter) as stabs/broth 5 ml as slants 3 ml It is prescribed not to exceed 60% of the total capacity of the container. Erlenmeyer flasks, media bottles, etc. *Volumes may vary depending on the laboratory practices.
- Large proteins travel/migrate faster in both gel filtration chromatography and SDS- PAGE. O True O FalseThe chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.