You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 10^5 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand? A) 1.64 x 10^5 cells B) 4.56 x 10^3 cells C) 5.23 x 10^10 cells D) 1.75 x 10^8 cells E) None of these

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 7.2P
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You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells.

Question: You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 10^5 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand?

A) 1.64 x 10^5 cells

B) 4.56 x 10^3 cells

C) 5.23 x 10^10 cells

D) 1.75 x 10^8 cells

E) None of these

Expert Solution
Step 1

The generation time is expressed as;

G=t/n

Where t is the time and n is the number of generations.

Through the equation 

Nt =No × 2n

number of cells can be calculated.

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