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- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.
- 1. List multiple reasons why preventing the growth of certain microbes is necessary/valuable 2. For bacterial growth, what can we do to compose a continuous culture where we would get continuous supply of the product? 3. Antibiotics - How did we compose major issues for treatment through resistance built up from the antibiotics?1. The technique used in bacteria for endospore staining can also be used to observe fungal spores. Select one: True False 2. Which of the following statements is/are TRUE? a. Agar does not provide nutrients to microorganisms. b. Peptone, pork extracts, and yeast extracts provide nutrients to microbes. c. Tap water must be used for preparing culture media. d. A & B are correct. e. B & C are correct Clear my choice 3. Motility of microorganisms is BEST observed with a a. Flagellar stain b. Hanging drop preparation c. Solid medium d. Negative stain e. Streak plate Clear my choice1. Compare the growth of the culture found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so? 2. Describe the abundance of the colonies found in the plates. What possible type of microorganisms are found in the plates?
- 2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)1. What kinds of media would be used to culture and Identify this microbe?2. What are some other potential microbes that could have this infection?1. Which of the following statements about agar is NOT true? A. It is derived from algae. B. it is typically utilized by bacteria. C. It is solid at room temperature. D. It is the defining ingredient between liquid and solid versions of the same media.
- Blood agar contains intact sheep red blood cells which are used to determine if a microbe has the ability to lyse these cells. This agar is properly termed A. a selective differential medium B. a complex medium C. a differential medium and a complex medium D. a selective medium48. Selective media is used to distinguish one microorganism type from another growing on the same media : A. False. B. True.3. Internet search for microbial colony descriptive terms to be used in your colony characterization. a. Whole colony shape b. Size of colony c. Edge/margin of colony d. Chromogenesis or pigmentation e. Opacity of colony f. Elevation of colony g. Surface of colony h. Consistency or texture of colony