The technique used in bacteria for endospore staining can also be used to observe fungal spores. Select one: True False 2. Which of the following statements is/are TRUE? a. Agar does not provide nutrients to microorganisms. b. Peptone, pork extracts, and yeast extracts provide nutrients to microbes. c. Tap water must be used for preparing culture media. d. A & B are correct. e. B & C are correct Clear my choice 3. Motility of microorganisms is BEST observed with a a. Flagellar stain b. Hanging drop preparation c. Solid medium d. Negative stain e. Streak plate

The technique used in bacteria for endospore staining can also be used to observe fungal spores. Select one: True False 2. Which of the following statements is/are TRUE? a. Agar does not provide nutrients to microorganisms. b. Peptone, pork extracts, and yeast extracts provide nutrients to microbes. c. Tap water must be used for preparing culture media. d. A & B are correct. e. B & C are correct Clear my choice 3. Motility of microorganisms is BEST observed with a a. Flagellar stain b. Hanging drop preparation c. Solid medium d. Negative stain e. Streak plate

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 2CR

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What is the best descriptive match for the following culture media? A. Specific biological characteristics allow this media to select specific groups of bacteria. B. All the components are well known. C. Will contain components that can inhibit certain groups of bacteria. D. Can be used to grow difficult growing or nutritional complex bacteria. E. This media contains amino acids for growing bacteria. select 1. Synthetic media select - 2. Selective media select - 3. Enriched media select 4. Differential media select - 5. Complex media
Which of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growth
2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.
The following media are used to grow bacteria for various purposes. Match the examples of media that best describe the various media. A. MacConkey agar B. Malt extract agar C. Blood agar D. Chocolate agar E. Stuart medium select - 1. Selective media select 2. Enrichment media select - 3. Differential media select - 4. Complex media select 5. Transport media
A. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?
Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture
1.You are given an unlabeled agar plate that is reddish in color. You do not know if it is MSA or MacConkey. What experiment would you do to find out? Explain what procedure you would do and what results you would expect to see on each plate to tell the difference.
1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.
7. Use the image to answer the following questions: (2) A student collects some of this bacteria to create a slide then performs a Gram stain as shown here: A. How many different types of bacteria are growing on the petri dish? B. How do you know (how can you tell the difference between the types of bacteria)? C. What is the Gram reaction and morphology of these cells? D. Based on the Gram stain, could this colony be used to grow pure culture? How do you know?
1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?
After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.
1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…