What is an example of a lab experiment that shows the variation of the photosynthesis efficiency in relation to different photic energy frequencies to which the reaction is exposed? Was it expected that green light frequency favored the reaction?
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- Researchers who study those rods cells have performed a series of experiments to better understand this phototransduction pathway and the role of this PDE protein in patients that suffer from night blindness. In one experiment, researchers have measured the cytosolic concentration of cGMP (cyclic- GMP) under different light intensities (exposure = 10 min) in both the presence and the absence of the PDE6i inhibitor. The results are as follow: the cytosolic concentration of cGMP (cyclic-GMP) decreases as light intensity increases, but only in the absence of the PDE6i inhibitor (see figure below). Without PDE6i cGMP concentration (A.U) 25 20 15- 10- 0 0 0.1 A.U (Arbitraty Unit) 0.2 0.3 Light intensity (A.U) 25 -20 -15 -10 -5 0.4 0 GMP concentration (A.U) cGMP concentration (A.U) 25 20- 15- 10 0- 0 0.1 With PDE6i 0.2 0.3 Light intensity (A.U) -20 C. The PDE protein is an oxidase that reduces GMP into a cGMP D. The PDE protein is a phosphodiesterase that converts cGMP into a GMP E. The PDE…Researchers who study those rods cells have performed a series of experiments to better understand this phototransduction pathway and the role of this PDE protein in patients that suffer from night blindness. In one experiment, researchers have measured the cytosolic concentration of cGMP (cyclic- GMP) under different light intensities (exposure = 10 min) in both the presence and the absence of the PDE6i inhibitor. The results are as follow: the cytosolic concentration of cGMP (cyclic-GMP) decreases as light intensity increases, but only in the absence of the PDE6i inhibitor (see figure below). Without PDE6i cGMP concentration (A.U) 25 20 15- 0- 0 0.1 0.2 0.3 Light intensity (A.U) 25 0.4 20 15 - 10 5 0 GMP concentration (A.U) cGMP concentration (A.U) 25 20 15- o 0- 0 0.1 With PDE6i 0.2 0.3 Light intensity (A.U) 0.4 25 20 0 GMP concentration (A.U) 10. What is the likely function of the cytosolic cGMP in this phototransduction pathway? A. It is a competitive inhibitor of PDE B. It is a…Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB? What is the equation for the double-reciprocal plot at 20 nMnM NAMB?
- What is the biochemical basis of optochin susceptibility testing?Since myoglobin is red and it may interfere with the absorbance of Bradford Protein Assay at OD595nm, how can you determine whether the effect of the interference is significant?When comparing MS2 spectrums of various bacteria, what specifically is it that you are looking for? Why are the peaks different and what does that indicate between different strains of bacteria?
- To further investigate the cellular response in photoreceptor cells, we will now look at the actual effect of cGMP within the phototransduction pathway. Again, we will compare what happens in the dark and in the presence of light to understand how an electric signal is generated in either condition. Comparing both conditions (in the dark or under the light), researchers have measured the molar concentration of cytosolic CGMP and recorded the membrane potential of rod cells by placing micro- electrodes on either side of their plasma membrane (see figure below). • A negative membrane potential means that there are more negatively charged ions inside the cell compared to outside the cell, or equivalently, that there are less positively charged ions inside the cell compared to outside the cell. • A positive membrane potential means that there are more negatively charged ions outside the cell compared to inside the cell, or equivalently, that there are less positively charged ions outside…To further investigate the cellular response in photoreceptor cells, we will now look at the actual effect of cGMP within the phototransduction pathway. Again, we will compare what happens in the dark and in the presence of light to understand how an electric signal is generated in either condition. Comparing both conditions (in the dark or under the light), researchers have measured the molar concentration of cytosolic cGMP and recorded the membrane potential of rod cells by placing micro-electrodes on either side of their plasma membrane (see figure below). A negative membrane potential means that there are more negatively charged ions inside the cell compared to outside the cell, or equivalently, that there are less positively charged ions inside the cell compared to outside the cell. ● A positive membrane potential means that there are more negatively charged ions outside the cell compared to inside the cell, or equivalently, that there are less positively charged ions outside the…Blood lactate is a biochemical by-product that is produced during high-intensity exercise due to insufficient oxygen intake. Commercially available drinks advertised as oxygen sup- plements claim to increase exercise performance and decrease the postexercise recovery period by decreasing the accumulation and accelerating the removal of blood lactate. To test this claim, researchers conducted a controlled experiment to compare the concen- tration of blood lactate during exercise and during recovery between two participant groups, one that ingested oxygen-supplemented water and another that ingested regular water. ( blood lactate (mmol/liter) C A B start с Exercise D middle -o-oxygen-supplemented water --regular water finish blood lactate (millimole/liter) b N The mean blood lactate concentration during exercise (left) and recovery (right) Which of the following best describes the results of the experiment? Recovery 3 6 9 12 15 18 21 24 27 30 time (mins) --oxygen-supplemented water…
- Blood lactate is a biochemical by-product that is produced during high-intensity exercise due to insufficient oxygen intake. Commercially available drinks advertised as oxygen sup- plements claim to increase exercise performance and decrease the postexercise recovery period by decreasing the accumulation and accelerating the removal of blood lactate. To test this claim, researchers conducted a controlled experiment to compare the concen- tration of blood lactate during exercise and during recovery between two participant groups, one that ingested oxygen-supplemented water and another that ingested regular water. blood lactate (millimole/liter) U A B start с Exercise D middle -o-oxygen-supplemented water regular water finish The mean blood lactate concentration during exercise (left) and recovery (right) Which of the following identifies the dependent variable in this experiment? the concentration of blood lactate the duration of the recovery period blood lactate (mmol/liter) the…The diagram shows a simplified cross-section of a human body and an ultrasound transducer.. 1swog meipsib eru of epnetster ritiW a) Explain what happens to the ultrasound signal as it travels from the probe through the body. b) Sketch a graph showing how the intensity of the signal detected by the probe varies with time following the transmission of a pulse of ultrasound with the probe in this positionThe maximum light absorption for the DNS assay:595nm240nm295nm540n