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- Practice question #7 chap. 2 p. 145 A. Explain the following observation: cells of E. coli fermenting glucose (anaerobic conditions) grow faster when NO; is supplied to the culture, and then grow even faster when the culture is highly aerated. B. Shiga toxin (Stx) is a potent A-B toxin that inhibits protein synthesis and has a lethal dose 50 (LD50) of approximately 20 ng/kg of body weight in rabbits (meaning that 1 µg of Stx would be lethal for half the people exposed to this dose). Nonetheless, cell lines derived from different mammalian tissues range from highly susceptible (cytotoxic dose 50 [CD50] of approximately 10 pg/ml) to completely resistant (CD50 > 1 µg/ml). What cellular or molecular differences do you think account for susceptibility and resistance of these cell lines? Note: LD50 is the dose at which 50% of the test subjects die. In this case, it was determined for rabbits given intravenous injections of the toxin. CD50 is the concentration at which 50% of the cells die.…Procedure #1: Treated with cholera toxins and adrenaline Procedure #2: Treated with nebivolol and adrenaline Which treatment will express very low or no activation of adenylyl cyclase in presence of adrenaline? Why?CELLULAR RESPIRATION AND FERMENTATION Name: Date: Instructor: Section/Group: POST-LAB QUESTIONS 1. Both cellular respiration and fermentation ordinarily begin with which molecule? ves 2. What role does cellular respiration play in the metabolism of an organism? 3. Glucose breakdown results in the breaking of C-H bonds and stored energy is released. Compare and contrast the end products of fermentation and cellular respiration in terms of their energy produced. 4. Fermentation results in the net production of only 2 ATP, while cellular respiration results in the production of at least 36 ATP. Explain these results with reference to the end products of both of these processes. Cellular Respiration and Fermentation 93
- Procedure #1: Treated with cholera toxins and adrenaline Procedure #2: Treated with nebivolol and adrenaline Part A: In what treatment will adrenaline cause a continual activation of adenylyl cyclase? Why?Will UPVOTE Answer the following: 1. What is the effect of boiling in the test tube with starch solution and saliva? 2. What is anaerobic oxidation? In your observation, which is the Hydrogen acceptor in the experiment, methylene blue or milk? 3. If the sample turned to blue or black upon the addition of I2 solution, is the honey genuine or not? Explain your answer 4. What changes do you think take place as the color changes upon the addition of phenol, pyrogallol or catechol and guac solution to the potato extract? 5. Differentiate an enzyme activator from an indicatorTrue or False 1. Cytochrome-mediated oxidation reactions are facilitated by microsomal enzymes. 2. Drugs bound to plasma proteins are ready for therapeutic action 3. Sublingual tablets, when administered , do not undergo absorption. 4. Generally, drugs with high lipid solubility are better absorbed into the bloodstream. 5. Urinary excretion of weakly basic drugs is enhanced by increasing the pH of the urine. 6. Most drugs are absorbed through active transport mechanisms. 7. Lying on the left side increases gastric emptying rate.
- 10:30 1 < 1. Short Answer 输入答案 Search ChP/USP/EP/BP..., find monograph of FLUOROURACIL; What kind of method used for "Impurities F and G" and "Related Substances" (+ Homework 2 O. .ll 4G 提交Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. 1. Why would furosemide help alleviate pulmonary edema? (2 sentences max)and the minimum exposure time 1. Determine the minimum inhibitory concentration given the following data: Concentration of ethanol Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) 10 5 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:
- Experiment Title: Cytotoxicity Test Experiment no. 2 Objectives: Study the eytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates. 1. Neerosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrcase in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die look for an adequa compound/condition (cancer and immotherapy) 2. Or you want to exclude cyto icity in specific cells (chemicals and drugs) 1 have a report on this subject, please solve Applications - Drug discovery proces - Oncological research - Safety evaluation of pesti ides, plantextracts, food additives, cosmetics, and my topic. industrial chemicals my Classification of cytotoxicity and cell viability assays These…Question;- Write the modes of action for the following measurements. There may be more than one measurement for each mode and/or more than one mode for each measurement. ■ Radioactive methionine incorporation ■ Radioactive uracil incorporation ■ Radioactive thymine incorporation ■ Glucose depletion ■ DNA present in the brothpls answer 4. Describe the two models that explain the behavior of allosteric enzymes. Include thelimitation or advantage of each. Give also an example of each.5. Explain the binding of oxygen to hemoglobin according to the two models in No. 4.