We need to prepare for our thesis work 75 Petri dishes with agar of mannitol (MSA). The recipe for 1L is in the following table:
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- We need to prepare for our thesis work 75 Petri dishes with agar of
mannitol (MSA). The recipe for 1L is in the following table:
a. Calculate how much is needed of every component to prepare the mL needed for 75 Petri dishes.
b. Calculate how much would be needed to prepare 50 Petri dishes.
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- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.Effect of pH on Microbial Growth 12 10 8 Escherichia coli -Pseudomonas aureus -Staphylococcus aureus -Listeria monocytogenes 1 4 pH Minimum Optimum Maximum Escherichia coli 4.5 7 Pseudomonas aureus 4.5 7.5 9.5 Staphylococcus aureus 6.8 Listeria monocytogenes 4.5 7 9.6 Make a discussion on the experiment of the effect of pH on microbial growth. Growth Rate of Microbial 2.Worksheet 5-1: Label Analysis-Lipids Instructions: Use the label for a frozen meal given below to answer the questions that follow on a separate sheet of paper. INGREDIENTS: COOKED FETTUCCINI Nutrition Facts Serving Size 1 Meal Servings Per Container 2 PASTA (WATER, SEMOLINA [DURUM WHEAT, NIACIN, FERROUS SULFATE, THAMINE MONONITRATE, Amount Per Serving RIBOFLAVIN, FOLIC ACID], EGG WHITE), COOKED CHICKEN BREAST Calories 670 Calories from Fat? % Daily CHUNKS (CHICKEN BREAST CHUNKS, Value* WATER, MODIFIED FOOD STARCH, Total Fat 43g ?% SALT, SODIUM TRIPOLYPHOSPHATE), Saturated Fat 17g Trans Fat 1g ?% BROCCOLI, HEAVY WHIPPING CREAM, WATER, CREAM CHEESE (PASTEURIZED MILK AND CREAM CHEESE CULTURES, SALT, CAROB Cholesterol 65mg Sodium 1180mg Total Carbohydrate 39g ?% BEAN GUM), SOYBEAN OIL, ROMANO (FROM COW'S MILK) AND PARMESAN CHEESE (PART-SKIM Dietary Fiber 6g Sugars 1g Protein 32g ?% MILK, CHEESE CULTURES, SALT, ENZYMES, CELLULOSE POWDER Vitamin A 15% Vitamin C 30% [PREVENTS CAKING]), HALF…
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- Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.Composition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02Aspergillus can be used to produce α-amylase (α-AM), which is an enzyme that hydrolyzes the glycosidic bonds in starch molecules into maltose (disaccharide). Pure α-AM has a specific activity of 56.00 U/mg. The α-AM is secreted into the culture media during production (after 5 days, production is complete). Following, the Aspergilluscells are separated by centrifugation and discarded. The culture supernatant (with α-AM) is filtered giving a clarified supernatant with a volume of 100.0 L, activity of 3.00 U/mL, and total protein concentration of 0.650 mg/mL. After purification, the product stream (7.50 L) contains α-AM (25.00 U/mL) and a total of 6.788 x 103 mg total protein. Calculate the following: Concentration of total protein (g/L) and total units of activity in the clarified supernatant and purification product stream. Specific activities (U/g) of the clarified supernatant and purification product stream. Consider significant figures in your answers (may want to use scientific…
- Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________Part a. You making 30 mo of agarose gel. Sybersafe A concentration 1000x. How much you will add......ul to the gel. b. You will be loading 20 your diagnostic digest onto your gal. The loading dye you have been provided is 5x concentrated. You will add..... ? ul to your sample before loading your gel.Ib 20 n moles OL produch ane observed In 10 minutes and 50 nmoles 20 minutes obsenved is Vo? product in are then cwhat
