value the cathode or anode). Protein eins wards Pepsin Lysozyme Hemoglobin Mynolohin IpH 1.0 11.0 6.8 pH 5.0 7.0 7.0 70 50
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- The plasma profiles of codeine (COD) and metabolites for 2 individuals (labeled A and B) are shown below. The X-axis is time in hours after an oral dose of codeine. [M=morphine; C6G=COD-6-glucuronide; M3G = morphine-3-glucuronide; NM (ignore)]. Note the data is shown on a log scale on the Y-axis. (A) Which individual is the poor metabolizer? Explain how you know this from the profiles? (B) Is this a problem for cough suppression? Explain. -CH HO Codeine COD 10 000 1000 C6G COD 100 M3G M6G NM 10 M 10 20 30 0 10 20 30 Plasma concentration (nmol I-)CYP2E1 has a much higher Km for ethanol than ADH (11 mM versus 0.05 mM,respectively). When greater portions of ethanol are ingested (0.08% or 17 mM), which part of the statement would be true?A sample of a protein is analyzed by CE using a neutral-coated capillary with a total length of 25.0 cm and a distance to the detector of 22.0 cm. Using an applied voltage of 20.0 kV, the protein was detected at a migration time of 16.2 min. The diffusion coefficient for the protein is 2.4 x 10-7 cm?/s. (a) Calculate the apparent electrophoretic mobility of the protein. (b) Calculate the expected number of theoretical plates for the analysis.
- The inner leaflet (monolayer) of the human erythrocyte membrane consists predominantly of phosphatidylethanolamine and phosphatidylserine. The outer leaflet consists predominantly of phosphatidylcholine and sphingomyelin. Although the phospholipid components of the membrane can diffuse in the fluid bilayer, this sidedness is preserved at all times. How?The following observations are made on an unknownmembrane protein, X. It can be extracted from disrupted erythrocyte membranes into aconcentrated salt solution, and it can be cleaved into fragments by proteolytic enzymes. Treatment of erythrocytes with proteolytic enzymes followed by disruption and extraction of membrane components yields intact X. However, treatment of erythrocyte “ghosts” (which consist of just plasma membranes, produced by disrupting the cells and washing outthe hemoglobin) with proteolytic enzymes, followed by disruption and extraction, yields extensively fragmented X. What do these observations indicate about the location of X in the plasma membrane? Do the properties of X resemble those of an integral or peripheral membrane protein?It is not an easy matter to assign particular func-tions to specific components of the basal lamina, sincethe overall structure is a complicated composite materialwith both mechanical and signaling properties. Nidogen,for example, cross-links two central components of thebasal lamina by binding to the laminin γ-1 chain and totype IV collagen. Given such a key role, it was surprisingthat mice with a homozygous knockout of the gene fornidogen-1 were entirely healthy, with no abnormal phe-notype. Similarly, mice homozygous for a knockout of thegene for nidogen-2 also appeared completely normal. Bycontrast, mice that were homozygous for a defined muta-tion in the gene for laminin γ-1, which eliminated just thebinding site for nidogen, died at birth with severe defectsin lung and kidney formation. The mutant portion of thelaminin γ-1 chain is thought to have no other functionthan to bind nidogen, and does not affect laminin struc-ture or its ability to assemble into the basal lamina.…
- Drag the labels onto the diagram to identify the anatomical terminology describing body orientation and direction (human a Posterior (caudal) Distal Superior (dorsal) Proximal Inferior (caudal) Anterior (ventral) Posterior (dorsal) Pearson archr^2=6Dt , where D is the diffusion coefficient of thediffusing object and t is the time that the object is allowed to diffuse. If the diffusion coefficient, D, of a small protein is 5 x 10^-10 m2 s-1, how long (on average) does ittake for the protein to diffuse across a parasitic wasp that is 189 um long ?A family eats homegrown vegetables daily which is irrigated with water from a river polluted with benzene having a concentration of 0.05 mg/L. The cancer potency factor for ingesting benzene is 2.9 x 10² mg/kg.day. a) Following the EPA exposure factors, determine the lifetime cancer risk assuming the receptor is an adult. State all assumptions. b) To reduce the lifetime cancer risk, it is proposed that the village replaces their homegrown produce with fish caught in the same river. Determine the percentage change in lifetime cancer risk from this potential solution and advise the community on the better food source of the two.
- Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation? use the following Figures 1and 2 respectively to explain pleaseHuman serum transferrin is an iron transport protein. With the use of appropriate diagrams, describe the metal-bound active site and discuss the structural changes that occur on iron binding to the protein.Electrophoresis is performed at PH 6.8 on a mixture of mutated hemoglobin that differ from normal haemoglobin (Hb) only by the substitution of one amino acid- Hb X: Val replaced par Glu - Hb Y: Asp replaced by Leu - Hb Z: Glu replaced by Lys What will be the order of migration between cathode and anode of these mutated Hb compared to normal Hb? Justify your answer.