Using the chromatogram find the flowing by calculating Rf: - The most and the least attractive component to Stationary phase? - The most and the least attractive component to Mobile phase? - If the target component has Rf = 0.08, which of the flowing material include the analyte? Solvent Front Distance solvent migrated = 5.0 cm 4.0 cm Distance A migrated = 3.ō cm Distance B migrated =2.0 cm 3.0 cm Distance C nțigrated = 0.8 cm 0.8 cm *--*--X- --X--X-- Origen A B U C D

Using the chromatogram find the flowing by calculating Rf: - The most and the least attractive component to Stationary phase? - The most and the least attractive component to Mobile phase? - If the target component has Rf = 0.08, which of the flowing material include the analyte? Solvent Front Distance solvent migrated = 5.0 cm 4.0 cm Distance A migrated = 3.ō cm Distance B migrated =2.0 cm 3.0 cm Distance C nțigrated = 0.8 cm 0.8 cm ----X- --X--X-- Origen A B U C D

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.12QAP

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