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- ADP and inorganic phosphate are provided for oxidative phosphorylation via an: ADP-ATP antiport and a proton-phosphate symport in the inner mitochondrial membrane. ADP-ATP antiport and a proton-phosphate symport in the outer mitochondrial membrane. O ADP-ATP antiport in the inner mitochondrial membrane and a phosphate uniport in the mitochondrial intermembrane space. ADP-phosphate symport in the inner mitochondrial membrane.(b) Metabolic state of cells and organism can be associated with inmplication of ATP and NADH. Explain this relationship.Sort the electron acceptors that are able to diffuse inside the mitochondrial matrix from those that are immobilized in a protein complex in their active state. Drag each item to the appropriate bin. • View Available Hint(s) Reset Help flavin mononucleotide flavin adenine dinucleotide nicotinamide adenine dinucleotide coenzyme Q iron-sulfur clusters cytochromes Able to diffuse into mitochondrial matrix Unable to diffuse into mitochondrial matrix
- The fatty acid side chains of the phospholipids in the inner mitochondrial membrane exhibit a large proportion of multiple unsaturations. As a result, will the membrane be hydrophobic or hydrophilic? Also will it be more fluid or more rigid? How do these characteristics help explain the observation that the inner membrane is impermeable to ions such as Mg2+ and Cl–? Hint: what moves these ions through the membrane?In the Electron Transport Chain:(a). Upon the transfers, what is the final recipient of these electrons?(b). What is the compound obtained upon the completion of electron transportation?(c). What causes the changes in pH between the inner mitochondrial membrane during the electron transfer?(A) What is the free energy yield associated with the transport of a proton from the mitochondrial intermembrane space (IMS) to the mitochondrial matrix, assuming that the pH of the IMS is 1.4 units lower than the pH of the matrix? (Hint: Calculate AGO' for the reaction H"IMS > H matris using the relationship AG° = RTInKeg and remembering that pH = -log1o[H*]. Assume that T = 310K (37°C), and R = 8.315x10-3 kJ mol-1 K-1. Show your work and include correct units!)
- Cyanide is a toxic substance. Cyanide is a cytochrome coxidase enzyme that is competitive. The last enzyme of the electron transport chain in mitochondria is cytochrome c oxidase. One of the most common substrates for cytochrome c oxidase is oxygen. Would an increase in the concentration of oxygen affect the inhibitioncaused by cyanide? How would conditions in the mitochondrial intermembrane spacechange if cytochrome c oxidase and the electron transport chain are notfunctioning properly. .Explain why this change in conditions will be harmful to a cell.Sonicating a suspension of mitochondria produces submitochondrial particles derived from the innermitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of themitochondria becomes the lumen of the submitochondrial particle. Explain (or diagram) the process of electrontransfer and oxidative phosphorylation in these particles. Assuming all the substrates for oxidativephosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH ofthe fluid in which the submitochondrial particles are suspended? Why?Although the outer mitochondrial membrane is permeable to all small molecules, the inner mitochondrial membrane is essentially impermeable in the absence of specific transport proteins. Consider this information answer: The ATP generated by oxidative respiration is used throughout the cell. The majority of ATP production occurs in the mitochondrial matrix. How do you think ATP is made accessible to enzymes in the cytosol and other organelles?
- 1. Name and draw diagrammatically the series of mitochondrial electron transfer catalysts, starting with the oxidation of NADH and succinate and ending with the reduction of O2. 2. Indicate the sites and stoichiometry (per 2e) at which protons are translocated from the matrix to the intermembrane space. 3. Indicate which complexes are inhibited by: amytal, antimycin A, azide (N3 ), cyanide (CN), carbon monoxide (CO), and rotenone. 4. Describe the effects of (1) oligomycin and (2) uncouplers of oxidative phosphorylation, e.g., dinitrophenol (DNP), carbonyl cyanide-p- trifluoromethoxyphenylhydrazone (FCCP), on respiration and ATP synthesis when added to a suspension of mitochondria with excess malate, ADP, and inorganic phosphate (Pi).In the 1930s, some physicians prescribed low doses of a compound called dinitrophenol (DNP) to help patients lose weight. This unsafe method was abandoned after some patients died. DNP uncouples the chemiosmotic machinery by making the lipid bilayer of the inner mitochondrial membrane leaky to H+. Chemical agents that cause this effect are called uncouplers. Explain how this could cause weight loss and also death. Considering the danger, is there any use for compounds like DNP or other uncouplers?Describe the involvements of the followings in the electron transport centre in the inner mitochondrial membrane.(a). Rieske center(b). Ubiquinone(c). Protons