The transcription factor Pax6 is required continuallyduring the life of a mouse (or a human) for the development and maintenance of the retina. HomozygousPax6 knockout mice die soon after birth because Pax6protein is also required in essential organs, such as thepancreas.a. In order to study the role of Pax6 in eye development, a researcher wants to generate a mouse thatexpresses Pax6 everywhere except in its eyes.Describe how you could construct such a mouse.b. Suppose the scientist wants to create a mousesimilar to that in part (a), but in which the eye cellsfrom which Pax6 function has been removed nowexpress GFP. Marking the cells in this way willallow the investigator to see the shapes of the Pax6−eye cells more easily than if they did not expressGFP. Diagram a Pax6 gene construct that wouldenable her to do this experiment.
The transcription factor Pax6 is required continually
during the life of a mouse (or a human) for the development and maintenance of the retina. Homozygous
Pax6 knockout mice die soon after birth because Pax6
protein is also required in essential organs, such as the
pancreas.
a. In order to study the role of Pax6 in eye development, a researcher wants to generate a mouse that
expresses Pax6 everywhere except in its eyes.
Describe how you could construct such a mouse.
b. Suppose the scientist wants to create a mouse
similar to that in part (a), but in which the eye cells
from which Pax6 function has been removed now
express GFP. Marking the cells in this way will
allow the investigator to see the shapes of the Pax6−
eye cells more easily than if they did not express
GFP. Diagram a Pax6 gene construct that would
enable her to do this experiment.
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