Q: What are the differences between a DNA library and acDNA library?
A: For the gene cloning method, the DNA is used, which is cut and ligated in the vector. This…
Q: Explain Proofreading by DNA polymerase.
A: A DNA polymerase is an enzyme that catalyze the synthesis of DNA molecules from nucleoside…
Q: Why isn’t cDNA synthetic
A: INTRODUCTION Deoxyribonucleic Acid, or DNA, is a molecule that holds the instructions that an…
Q: Why Sanger Sequencing Dependson DNA Polymerase?
A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…
Q: could Nirenberg and Matthaei have substituted RNA polymerase instead of polynucleotide phosphorylase…
A: The scientist Marshall Nirenberg and Heinrich J. Matthaei designed an experiment to elucidate the…
Q: Why the DNA sequencing alone is often not sufficient to produce a genome assembly of desired…
A: DNA sequencing is basically the process by which the nucleic acid sequence can be determined. the…
Q: What is meant by “proofreading” with respect to DNA polymerase?
A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…
Q: The proofreading function of DNA polymerase involves the recognitionof a ________ and the removal of…
A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…
Q: DNA primase synthesizes a short RNA primer that later appears at the 5'-end of each Okazaki…
A: The given statement is TRUE
Q: Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In…
A: DNA replication and Polymerase Chain Reaction are both processes that create copies of DNA. DNA…
Q: TaqMan probes can specifically measure human DNA concentration, but SYBR Green I cannot-explain.
A: Biotechnology is technology that utilizes biological systems, living organisms or parts of this to…
Q: Explain how DNA polymerase III adds dNTPs onto the RNA primer.
A: DNA Polymerase: A DNA polymerase is an enzyme that catalyses the manufacture of DNA molecules from…
Q: The following sequence of nucleotides is found in a single-stranded DNA template:…
A: The process of formation of mRNA from DNA is called transcription.
Q: How does proofreading improve replication fidelity?
A: Proofreading is a mechanism by which DNA polymerase improve the fidelity of DNA replication.…
Q: How does proofreading take place in the process of DNA replication?
A: DNA replication is the process of producing two identical replices of DNA from one original DNA…
Q: The polymerase chain reaction can only be used to amplify genes that have been cloned and sequenced.…
A: The polymerase chain reaction was originally developed by the American biochemist Kary Mullis. The…
Q: Describe the role of dideoxyribonucleotides ingenerating DNA fragments for analysis
A: Sanger developed a method to determine the sequence of deoxyribonucleic acid (DNA) called the Sanger…
Q: Why is it a large undertaking to construct a DNA library?
A: A deoxyribonucleic acid library could be an assortment of cells that carry cloned items of the whole…
Q: Compare DNA polymerase, RNA polymerase, poly(A) polymerase, and CCA-adding polymerase with respect…
A: Polymerases are enzymes help in the long chin of nucleic acid synthesis. Deoxyribonucleic acid (DNA)…
Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…
A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…
Q: In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?
A: Ribonucleic acid sequencing (RNA-seq) was developed by Michael Snyder and colleagues in 2008. It is…
Q: Explain the proofreading function of DNA polymerase.
A: Proofreading means correcting the incorrect base pairs by removing and adding correct base pairs.…
Q: Explain the mechanisms on the proofreading of DNA.
A: Biological macromolecules are those large molecules that are necessary for the survival and growth…
Q: Does proofreading always take place by the same process in replication?
A: REPAIR PATHWAYS:- Both in Prokaryotes and Eukaryotes, there is a repair enzyme system to deal with…
Q: Explain why DNA polymerase requires a template and a primer.
A: DNA replication is a process, in which the DNA polymerase (DNA pol) is the main key proteins that…
Q: You are working to optimize CRISPR. How could you use ChIP-Seq to inform your design of guide RNA?
A: CRISPR-Cas9 mechanisms recognize DNA targets that are complementary to a short CRISPR RNA (crRNA)…
Q: Although RNA polymerase is a processive enzyme that remains attached to the DNA over long stretches…
A: RNA polymerase is an enzyme that assists in the transcription process by copying a DNA sequence into…
Q: Why is RNA produced only from the template DNAstrand and not from both strands?
A: The fundamental process of gene expression is transcription. Coping of information from DNA to RNA…
Q: Why can’t a researcher use a poly-T primer in a reverse transcriptase reaction that utilizes…
A: Introduction :- Reverse transcriptase is mRNA dependant DNA polymerase enzyme. It is the type of…
Q: "Polymerase binding is never very efficient unless CAP is alsopresent to facilitate the process".…
A: A functional unit of DNA comprising a cluster of genes controlled by a single promoter is called an…
Q: Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain…
A: The polymerase chain reaction (PCR) is the process of artificially synthesizing multiple copies of a…
Q: Explain the roles of DNA polymerase, the template, and the primer in a Sanger sequencing reaction?
A: Genome Sequencing is total set of genes present in an organism. It contains millions to billions of…
Q: Ruwaifa want to compares a nucleotide query sequence what option he will opt in BLAST and why?
A: BLAST is Basic local alignment search tool used to compare the queries in a DNA database. They are…
Q: Which of the following subunits of DNA polymerase has proofreading ability?
A: Introduction: The hereditary material which transfers from parents to offspring is DNA. It is stored…
Q: What would happen if DNA Polymerase II is mutated? Elaborate. Differentiate the Shine-Dalgarno…
A: DNA Polymerase II helps in the repair of damaged or mismatched nucleotide base pairs. If the DNA…
Q: Earlier, proofreading DNA polymerases used to have higher fidelity than that of Taq DNA pol. but…
A: PCR is polymerase chain reaction. It was used initially for polymerization of the small fragments of…
Q: Briefly explain why RNA-seq gives more information about the transcriptome than does microarray…
A: The RNA transcripts obtained after DNA are converted into RNA by several enzymes by transcription is…
Q: "Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries". Explain how ?
A: Introduction The cDNA library is a collection of mRNA segments cloned into independent vector…
Q: What is an advantage of RNA Seq over microarrays?
A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…
Q: Why the concept of a DNA library is still important for a number of modern applications ?
A: A DNA library can be described as the collection of DNA fragments that are kept and propagated in a…
Q: What is the meaning of proofreading activity ? O A. The polymerase checks for the correct…
A: DNA means deoxyribo nucleic acids. DNA act as genetic material in most organisms present on earth.…
Q: Describe the proofreading activity of DNA polymerase.
A: Although DNA polymerase is a very effective enzyme, it can potentially introduce an erroneous…
Q: Briefly explain why RNA-seq gives more information about the transcriptome than does microarray…
A: Messenger (m-RNA) ribonucleic acid is known to be transcribed from the DNA molecule, which is the…
Q: Explain how DNA is sequenced by the Sanger chain termination method
A: DNA is a polymer of multiple nucleotides connected together via phosphodiester bonds. A nucleotide…
Q: Since the blue indicate the number of sequenced RNA samples what does the purple bars indicate in…
A: RNA are formed by the transcription of DNA. The main enzyme for transcription is RNA polymerase that…
Q: Let’s say that you want to find out the difference in nucleotide sequence among two DNA strands, one…
A: DNA sequencing is a technique used to study the nucleotides of DNA. It is used to determine the…
Q: Why can both DNA template strands not be replicated continuously.
A: Deoxyribonucleic acid (DNA) is a molecule that contains the biological instructions that make each…
The major products of proofreading by RNA polymerase are dinucleotides rather than mononucleotides. Why?
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- What is meant by “proofreading” with respect to DNA polymerase?Explain the mechanisms on the proofreading of DNA.A single (+) strand of DNA (base composition: A, 21%; G, 29%; C, 29%; T, 21%) isreplicated by DNA polymerase to yield a complementary (−) strand. The resultingduplex DNA is then used a template by RNA polymerase, which transcribes the (−)strand. Indicate the base composition of the RNA formed.
- A Sanger product of the sequencing of a template DNA is presented +ddTTP +ddATP +ddCTP +ddGTP Mononucleotide Pentanucleotide Trinucleotide Dinucleotide 11-nucleotide Hexanucleotide Octanucleotide Tetranucleotide 16-nucleotide Decanucleotide Nonanucleotide Heptanucleotide 17-nucleotide 15-nucleotide 13-nucleotide 12-nucleotide 18-nucleotide 20-nucleotide 14-nucleotide 19-nucleotide 21-nucleotide 1. Determine the sequence of template DNA 2. Determine the mRNA sequence from this template DNA 3. Determine the the sequence of protein product derived from that DNAa) Explain how the molecular mechanism of DNA polymerase enhances DNA replication. b) Discuss the characteristic of DNA polymerase 1, Nick translation ProofreadingIn the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?
- A single (+) strand of DNA (base composition: A, 21%; G, 29%; C, 29%; T, 21%) is replicated by DNA polymerase to yield a complementary (-) strand. The resulting duplex DNA is then used a template by RNA polymerase, which transcribes the (-) strand. Indicate the base composition of the RNA formed.To amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the tube is 1) a buffer solution, 2) the DNA you want to amplify, 3) some DNA nucleotides, 4) a polymerase (like Taq polymerase), and O an RNA polymerase a set of forward and reverse primers some phospholipids for a cell membrane some ribosomesLet’s say that you want to find out the difference in nucleotide sequence among two DNA strands, one of which is isolated from the liver of a liver cancer patient and the other one is isolated from the liver of a healthy individual. How you can do that, please explain in details