The hypothesis for the experiment shown in the picture  is that the TamC3 and TamR3 cell lines function, and grow the absence of estrogen, receptor function. Reduced reliance on estrogen, receptor signaling alters mTOR pathway activity. If this hypothesis is true, then mediators of mTOR signaling (Akt or p70S6K) should have reduced levels of phosphorylation . A. Where would you look to see this? B. To what degree does the data in the picture support the hypothesis?

Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter5: Abbreviations And Systems Of Measurement
Section: Chapter Questions
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The hypothesis for the experiment shown in the picture  is that the TamC3 and TamR3 cell lines function, and grow the absence of estrogen, receptor function. Reduced reliance on estrogen, receptor signaling alters mTOR pathway activity. If this hypothesis is true, then mediators of mTOR signaling (Akt or p70S6K) should have reduced levels of phosphorylation . A. Where would you look to see this? B. To what degree does the data in the picture support the hypothesis?
MCF-7
TamC3
TamR3
P-Akt
Total Akt
P-p7056K
Total p7056K
actin
Transcribed Image Text:MCF-7 TamC3 TamR3 P-Akt Total Akt P-p7056K Total p7056K actin
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