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- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantprocedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
- use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30Detail of Pfizer-BioNTech i.e. vector used etcВackground In order to determine whether a newly synthesized chemical might be a useful food preservative, the chemical was tested for its ability to inhibit bacterial growth. Control. 500 ml of cottage cheese was inoculated with 2 ml of a 24-hr culture of Pseudomonas aeruginosa and incubated at 25°C. Five hours after inoculation, a standard plate count showed there were 200 bacterial cells/ml in the cottage cheese. After 11 hours, 1600 cells/ml. After 21 hours, 50,000 cells/ml. After 27 hours, 400,000 cells/ml. Experiment. 500 ml of cottage cheese containing the preservative was inoculated with 2 ml of a 24-hr culture of P. aeruginosa. After 6 hours of incubation at 25°C, a standard plate count was performed. There were 700 bacterial cells/ml in the cottage cheese. After 14 hours, 11,000 cells/ml. After 20 hours, 90,000 cells/ml. After 28 hours, 1, 400, 000 cells/ml. Use the data and the semi-log graph paper to plot growth curves for the control and the experiment. Remember to label…
- The thermal death point is best described as:(a) The highest temperature that will support growth of amicrobial culture(b) The length of time required for killing 100% of mi-crobes in a 24-hour culture that is heated to 100°C(c) The temperature at which a previously growing 24-hourculture will begin to die(d) The temperature at which 100% of the microbes in a24-hour culture will be killed in 10 minutesGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3
- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Describe how you would execute this first stage from the point you are handed the nutrient broth tube containing the mixed culture, through to the appearance of two colony types on a nutrient agar plate. Assume you have all the necessary equipment and materials at your disposal. Be concise, but thorough; limit yourself to a short paragraph (1/4-1/2 page at most) – include the method and techniques; what you expect to see, and how you would avoid contamination during the process.After a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE: