Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a 10^-2 dilution of the entire culture. Explain how you would do this. Show your calculations.
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Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a 10^-2 dilution of the entire culture. Explain how you would do this. Show your calculations.
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- Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?The replicate results (mg/L) obtained from calcium determination of a blood sample using atomic absorption spectrophotometry (AAS) and a new colorimetric method were as following: AAS : 11.2, 11.2, 10.6, 11.2, 9.7 and 10.0 Colorimetric : 9.2, 10.5, 9.7, 11.5, 11.6, 9.3, 10.1 and 11.2 Determine whether the mean of both methods have a significant difference at 95% confidence level.The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.To prepare a vaginal irrigation, a nurse uses 3 tsp of vinegar and 1.5 L of water. a. How many ml of vinegar should she use? b. If she prepares a solution using only 500 ml of water, how many ml of vinegar should she use?Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml blanks. Show how you would plate out 102 through to 10-6
- You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:(The top set of images are photographs of your results for MSA and MAC. The bottom set of images are illustrations that reflect the results you should have observed in the photographs.) Culture #:[Type here] Organism:[Type here] Name: Combination Media: Mystery Organism Identification You will be given a pure culture of one of three organisms. Your assignment is to identify which of the three possibilities is in your culture tube. To do this, you will use two different types of media: Mannitol Salt Agar and MacConkey's Agar. Complete the table below by predicting the reactions of each organism on each medium (Growth or no growth? If you expect growth, what color should it be?). Do this before coming to lab on the due date. The information about each organism below and the information found in the theory sections for the labs should help you make your predictions. If you complete this table correctly, it will be a big help to you while you try to identify MSA MAC your mystery organism!…
- 1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul
