Select the correct order of Rf values that you expect when monitoring the progress of the saponification reaction by TLC, using 1:1 Hexane/Ethyl acetate as the developing solvent. Rf (carboxylic acid)>Rf (alcohol)> Rf (ester) Rf (ester>Rf (carboxylic acid) > Rf (alcohol)) O Rf (ester)>Rf (alcohol)>Rf (carboxylic acid)
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- Which layer (aqueous or organic) will naphthalene partition into during the extraction process? 3M HCL or Ethyl AcetatePease indicate the compound that best fits in the blank of the separation scheme below. OH H2N Benzoic Acid Biphenyl Benzocaine Phenol benzocaine phenol biphenyl benzoic acid dissolve in ether; extract with aqueous 19)_ ether aq 20) CO:(g) H2O HIDDEN COMPOUND 1 HIDDEN COMPOUND 2 HIDDEN COMPOUND 3 trace 21) |6.0M М НСI aq NaCl 22). HO Which of the following is the best choice for blank 21 in the given separation scheme? O H2SO4 O pentane O HCI NaHCO3 O H20Qs4. This is a TLC analysis of 3 individual compounds. The plate was run in 20 % ethyl acetate, 80% hexane solvent mixture (eluent). Draw a schematic TLC plate for the same compounds, but when the eluent is 40% ethyl acetate, 60 % hexane. Indicate, what is the most polar and least polar compound (A, B, or C) -- A B C
- A semi-purified crude extract(SP) was tested against five known phenolic compounds, namely: (1) Quercetin, (2) Rutin, (3) Gallic acid, (4) Catechin, and (5) Ellagic acid. Answer the following questions: Based on the developed chromatogram, what can you infer about the semi-purified crude extract? Based on the developed chromatogram and your knowledge about TLC, what can you say about compounds 1 and 2? Is TLC the most accurate and reliable chromatographic technique used in analytical chemistry? If yes, why? If not, what is a more accurate and reliable chromatographic technique than TLC?Table 1: Selected phytocomponents identified in the plant extract. Retention time, min Name of Compound 10.767 Benzaldehyde 15.790 Azulene 16.002 1-Piperazinecarboxaldehyde 17.935 Phthalan 20.731 2-methoxy-1,3,4-trimethybenzene 24.028 Methylesterpentanoic acid 26.993 Tetradecamethylcycloheptasiloxane 31.753 Hexadecamethyl Cyclooctasiloxane If GC is combined with MS, what data you will add as the 3rd column in Table 1 above. Fill in that data for each of the select identified phytocomponents.g) h) i) j) H₂C Ph OCH3 HO OCH₂Ph OSI(CH₂)3 Br PPha + 2 1) "Buli /THF / rt/1.5 h 2) OsO4/ NalO4 acetone /ether LOCH3 -Si(CH3)3 CH3 /nt/1 h CH₂Cl₂-78°C / 15 min PPn, /1₂ (1 eq) imidazoie / THE 0°C - rt
- 6. Discuss briefly why the following TLC experiments were not performed properly A B 7. Arrange the following compounds in order of increasing the polarity: CH3(CH2),0CH3, CH;(CH2)20H, CH;(CH2);CO2H, CH;(CH2)«CH3, CH3(CH2);COH6) What is the nucleophilic site in the following compounds? H3C-0-CH3 H2C=CH2 CH;NH2 II II A) | = Hydrogen; I| = TT electrons in bond; III = nitrogen. %3D B) | = Oxygen; II = carbon; III = nitrogen. C) Hydrogen; II = carbon; III = carbon. %3D D) | = Oxygen; II = TT electrons in bond; III = nitrogen.To determine the purity of a synthetic preparation of methylethyl ketone, C4H8O, it was reacted with hydroxylamine hydrochloride, liberating HCl (reaction: NH2OH.HCl + C4H8O -> C4H9NO + H20 + HCl). In a typical analysis a 3.00-mL sample was diluted to 50.00 mL and treated with an excess of hydroxylamine hydrochloride. The liberated HCl was titrated with 1.2149 M NaOH, requiring 35 mL to reach the end point. A. Write the acid base reaction involved in the titration. B. Report the percent purity of the sample given that the density of methylethyl ketone is 0.805 g/mL.
- R. 10. To extract benzoic acid from a mixture of aniline, and methoxybenzene, we follow the steps, dissolve mixture NaOH(aq) organic layer in ether aqueous layer b) dissolve mixture HCl(aq) in ether dissolve mixture in ether d) dissolve mixture in ether H₂O H₂O organic layer aqueous layer organic layer aqueous layer organic layer aqueous layer separation separation separation separation aqueous layer HCI. Cone precipitaion and filtration aqueous layer NaOH(aq) organic layer aqueous layer precipitaion and filtration evaporation precipitaion and filtrationcan i get the answers 1-3 from this from TLC organic chem ResultsContains all relevant results.ResultsContains all relevant results. 1= Menthol 2= Menthone 3= Limonene 4- Cymene HO menthol menthone limonene mene 1. A plate which shows how all 4 compounds would migrate relative to each other) 2.3 plates showing each of the three stains 3. Include a table that states all distances between baseline and spots, and baseline and the solvent front, as well as the Rf for each spotA mixture of tert-butylbenzene and acetophenone is adsorbed onto a silica gel chromatography column and eluted with a moderately polar solvent such as dichloromethane. Match each statement to the appropriate category. least polar tert-Butylbenzene elutes first most polar elutes last Answer Bank Acetophenone