QUESTION 2 Which of the following errors would result in positive results for all samples in an ELISA assay (including negative co O Failure to perform the wash step after incubation with secondary antibody. O Failure to perform the wash step after initial incubation with controls/samples. O Failure to perform the wash step after incubation with primary antibody. QUESTION 3 Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? O Failure to add antigen. O Failure to add funtional primary antibody. O Failure to add functional secondary antibody. O Failure to add functional substrate. QUESTION 4 Reusing a pipet tip when transferring controls and samples from tubes to wells is unlikely to cause false negatives or false positives. is more likely to cause false positives than false negatives. is more likely to cause false negatives than false positives. O is likely to cause both false positives and false negatives.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter4: Basic Immunology And Immunohematology
Section4.3: Tests For Rheumatoid Factors
Problem 2.1CS
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QUESTION 2
Which of the following errors would result in positive results for all samples in an ELISA assay (including negative controls)?
O Failure to perform the wash step after incubation with secondary antibody.
O Failure to perform the wash step after initial incubation with controls/samples.
O Failure to perform the wash step after incubation with primary antibody.
QUESTION 3
Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? Select all that apply.
O Failure to add antigen.
O Failure to add funtional primary antibody.
O Failure to add functional secondary antibody.
O Failure to add functional substrate.
QUESTION 4
Reusing a pipet tip when transferring controls and samples from tubes to wells
O is unlikely to cause false negatives or false positives.
is more likely to cause false positives than false negatives.
is more likely to cause false negatives than false positives.
O is likely to cause both false positives and false negatives.
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Transcribed Image Text:QUESTION 2 Which of the following errors would result in positive results for all samples in an ELISA assay (including negative controls)? O Failure to perform the wash step after incubation with secondary antibody. O Failure to perform the wash step after initial incubation with controls/samples. O Failure to perform the wash step after incubation with primary antibody. QUESTION 3 Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? Select all that apply. O Failure to add antigen. O Failure to add funtional primary antibody. O Failure to add functional secondary antibody. O Failure to add functional substrate. QUESTION 4 Reusing a pipet tip when transferring controls and samples from tubes to wells O is unlikely to cause false negatives or false positives. is more likely to cause false positives than false negatives. is more likely to cause false negatives than false positives. O is likely to cause both false positives and false negatives. Click Save and Submit to save and submit. Click Save All Answers to save all ansuers. 10 Type here to search
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