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Genetics Q1
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- Question 17 What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3 A 5'-UAGUGACAGUUGCGAU-3' B 5-TAGCGTTGACAGTGATA-3 5'-ATCACTGTCAACGCTA-3 D 5'-TAGTGACAGTTGCGAT-3'Question 46 Cellular transcription is : O Use of DNA as a template to make complementary DNA Use of DNA as a template to make complementary RNA O Use of RNA as a template to make proteinsQuestion #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…
- Question 7 (2 points) Determine the matching base pairs that RNA polymerase would lay down for this DNA sequence: TGCAGACT *record your answer with no spaces, dashes, or other charactersQuestion 43 The DNA strand that serves as a template during transcription is known (other than "template") as the or noncoding strand. Blank 1 Blank 1 Add your answerQuestion 19 A transversion mutation would be replacing T by: A either A or G BU (c) T D) c
- QUESTION 17 OL NEere interested in generating a PCR amplicon including the bracketed sequence below. Which of the following sequences would be canen hybridizing (annealing) with the target AND would also serve to generate a copy of the bracketed region of interest? 5'-AATCGT[AGCAGCAGCAGTGGCT]A AGCT-3 3' -TTAGCA[TC GTC GTC GTC ACC G A] TTCG A - 5' 3-TTAGC-S S-AATCG-3 OSAAGCT-3 5-AGCTT-3 5-GCTAA-3 5-TCGAA-3 QUESTION 18 Vhich of the following is/are true regarding the enzvme PRIMASE? Save and Submit to save and submit. Click Save All Answers to save all answers.Question 47 (a) Use the following figure to determine the changes to the amino acids that correspond to the normal and mutated DNA sequences. Normal DNA sequence: 3 CAT TCA AAC ATT 5 Mutated DNA sequence: 3 CAT AGT GAG GTC 5 (Hint: Write the mRNA first, then identify the amino acids.) (b) What type of mutation is shown? First base of codon U C A G U UUU UUC UUA UUG CUU CUC CUA CUG AUU AUC AUA AUG GUU GUC GUA GUG UCU UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA Met ACG GCU GCC GCA GCG Phe -Leu Leu lle Second base of codon A C Val -Ser Pro Thr Ala UAUTYT туг UAC UAA UAG CAU CAC CAA CAG. His Gin AAU AAC Asn AAA GAUT GAC GAA GAG Asp G AAGLYS AGG. GGU GGC GGA GGG Glu UGU UGC UGA UGG CGU CGC CGA CGG Bys A Trp G U C -Arg AGU AGC AGA аса за Ser Arg U C Gly AG A U C A G U C A G Third base of codonQuestion 33 What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGCCCGTATAC -5 O 3- GCCTACGGGCATATG -5 O 5- GCCTACGGGCATAAG -3 O 5- GCCTACGGGCATATG -3 O 3- CGGATGCCCGTATAC -5
- Quèstion 34 Enzyme that joins the DNA fragments O Recombinant DNA Technology O Restriction enzymes O Ligase O Palindromic sequencesQUESTION 10 You grow this weird looking organism in lab and have no idea what it is. You decide to sequence its genome and one of the reads you get back is shown below: >UnknownSequence1 GCGGTATTTGACGCCGCATTCGAAGTGGTCGATCCATTCGGCGTATCAGGACAATTTGCATATGTTGACGTTGTCGCGGGGTGGGCCCTCGATCTGGATGTCGGAGCGGGACGCG GCGAAGATCGGTGTGGCCGATAATGATTGGATCGAGGCGGTCAATCGTAACGGGGTGGTGGTGGCGCGGGCGATCGTGTQuestion 16 Why can't SNPS be detected by PCR and Gel Electrophoresis? O Because Gel Electrophoresis detects size differences in DNA and SNPS do not change the size of the DNA strand. O Because SNPS cause deletions so large that they are beyond the limits of this technique to detect. O Because SNPS affect proteins and PCR only works on DNA. O Because SNPS are too complicated to detect with this technology.