Question 1ListenIf you are given a sample with 1,000,000 pfu/mL of bacteriophage (Tube A) and plate 1mL of the sample on a TSAplate, what will you observe after 24 hours incubation? (How many plaques?)100,000 because it is a 1:10 dilution.Zero because bacteriophage need a host to grow.More than 300 because it will be TNTC.1,000,000 plaques because there are 1,000,000 phage in 1mL.

Understanding the relationship between the initial phage concentration, dilutions, and plaque…

Answered: Question 1 Listen If you are given a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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QUESTION 1 The table below shows the results from looking at the diagnostic accuracy of a new rapid antigen test for COVID-19 in 100000 patients, compared to the reference standard RT-PCR test. The rows of the table represent the test result and the columns the true disease status (as confirmed by RT-PCR). COVID-19 Positive COVID-19 Negative Antigen Test Positive Antigen Test Negative 424 795 8216 90565 Calculate the SENSITIVITY of this new rapid antigen test as percentage with 3 decimal places. (Assume that the RT-PCR reference has a 100% Sensitivity and 100% Specificity.)
Question 7 On which of these plates would you be most surprised to see colonies of E. coli growing after incubation? 1. antibiotic resistant E. coli on streptomycin positive plate 2. antibiotic resistant E. coli on streptomycin negative plate 3. antibiotic non-resistant E. coli on streptomycin positive plate 4. antibiotic non-resistant E. coli on streptomycin negative plate
QUESTION 5You are asked to recommend an antibiotic to treat potential pathogenicityt in an animal. Describe a method which will allow you to make this recommendation.
QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O Xgal
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
QUESTION 11 A graduate student, whom had not taken BIOL 531, localized the epitope for an antibody generated by the COVID19 vaccine to the peptide sequence below. S21P2 PSKPSKRSFIEDLLFNKV The student did a Blast search of the human proteome and found matches to human proteins. They are concerned that this means the vaccine could cause an autoimmune rection to human proteins. Is this student justified in this concern? Use statistics from a Blast search to justify your answer (Limit 5-6 sentences).
Question 7 A series of mutant strains (a through f) of a particular mold are not able to grow on minimal media unless they are supplemented by one or more of the compounds Q through V as shown below. Strain a b с d e f Q 0 0 + 0 0 + R 0 + + 0 + + Supplements S 0 o 0 0 0 0 T + + + + + + U V 0 + + 0 0 + 0 + + + + + + = growth 0 = no growth Draw a plausible metabolic pathway supported by the data provided in the Table above, showing the position of each metabolic intermediate in the pathway, which step is blocked by each mutant and explain your answer. (8 marks)
QUESTION 1 Questions 1-5. Please fill in the blank below with the corresponding structures indicated below in the diagram: Please ignore the fact that the fragments are colored blue and green. The coloration has no significance to this question. Also, please don't forget to answer question 61 1. Leading strand= 2. Lagging strand= 3. A single Okazaki fragment= 4. 3' end of fragment labeled "H"= 5. Replication fork= H E G 6. How many primers are required to replicate all of the DNA fragments in the above diagram (include both lagging and leading strand)?=
QUESTION 11 Below is a diagram of an assay you learned a lot about in class. In the third image, what is the little purple ball near the top supposed to represent? 1 O Analyte O a patient sample O An enzyme O A substrate A virus O An antibody 2.
Question 6 In the first part of the experiment, there were only a few colonies on the streptomycin positive plate. In the second part, there are a large number of colonies on the streptomycin positive plate that was inoculated with the antibiotic resistant strain. What is the most likely explanation for this difference? 1. The antibiotic resistant strain was composed of a large number of individuals with resistance to streptomycin 2. The exposure to streptomycin caused a mutation in a few individuals
QUESTION 13 A scientist would like to overexpress a lysosomal acid hydrolase in a cultured cell line as a way to investigate a potential therapy for a lysosomal storage disease. How might this scientist introduce a plasmid encoding this lysosomal enzyme into cells? O Scanning electron microscopy O Western blot analysis O Transfection with a lipid reagent (AKA liposomes) O Immunohistochemistry O Generate an organoid QUESTION 14 Heterotrimeric G-proteins that associate with the G-protein coupled receptors (GPCRs) consist of which of the following? OA. beta subunits C B. O C. alpha subunits alpha, beta, and gamma subuni OD. beta and gamma subunits O E. gamma subunits QUESTION 15 The activation of a common effector molecule in the cytoplasm by signals from a variety of unrelated receptors is called OA. Divergence OB. Convergence OC. Crosstalk OD. Transvergence QUESTION 16
QUESTION 1 SDS-PAGE reagents that play a role in formation of the gel only are(Select all that applies) Beta-Mercaptoethanol Bromophenol blue APS Heat Sodium Dodecyl Sulfate Bis-acrylamide Acrylamide TEMED 3.33 points QUESTION 2 In Lab 3 we aimed to separate lysozyme from the Hen egg white proteins. Which of the following is the correct observation/result from that lab? Load contains all the neutral and negatively charged proteins. HEW contains negative and neutral proteins only. Carb 1 contains negatively charged proteins. Load contains positive, negative and neutral proteins. 3.34 points QUESTION 3 In order to separate negatively charged proteins from a mixture of neutral, positively charged and negatively proteins, you should pass the mixture of these beads through Gel filtration column, using anion…

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