Write a short discussion based on the graph and calculate mean growth rate and mean generation time ProcedureAAdd50 ulofTake 3 mlmixtureMixSmlTop up to S0ml,to - Add fresh LB ampicillin totheovernight culture-mixturewith 45 ml LaanalysethebothbrothOD600Withdraw 3ml mixture toThe mixture is thenat shakedMeasure every 15mins. Stop until incubated75 mins.Overnight culture(E-coli LB borth)analyse (OD600)record aszero time-point.incubator at 37°c 200rpmMixovernight cultureB (control) with 45 ml LBSmlTake 3 mlAdd 50 ul ofampicillin to themixtureanalyseO0600toNo ampicilinthemixturebrothMeasure every 15mins. Stop until75 mins.The mixture is thenincubated atWithdraw 3ml mixture toanalyse (OD600)record aszero time-point.shaked-incubator at 37c 200rpmGraph of Absorbance vs Time(mins)1.41.20.80.60.40.21020304050607080Time (mins)Group 1OD 600(A)(B)Group 1 (Ampicilin)Group 1 (Control)Ampicillin0.124ControlTime (minutes0.248150.7560367300.6450.428450.6330.695600.5990.937750.5931.301Absorbance(600nm)

One of the ways of measuring bacterial growth is the measurement of the optical density of the…

Procedure A Take 3 ml Top up to S0ml, Add 50 of Mix Sml mixture analyse to - Add fresh LB ampicillin to the mixture overnight culture- with 45 ml LB the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. Overnight culture analyse (OD600)record as zero time-point. - incubated at shaked (E-coli LB borth) incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Add 50 ul of ampicillin to the Take 3 ml mixture to No ampicilin analyse O0600 the mixture broth The mixture is incubated incubator at 37c 200rpm Withdraw 3ml mixture to analyse (OD600)record as zero time-point. then Measure every 15 mins. Stop until 75 mins at shaked

Procedure A Take 3 ml Top up to S0ml, Add 50 of Mix Sml mixture analyse to - Add fresh LB ampicillin to the mixture overnight culture- with 45 ml LB the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. Overnight culture analyse (OD600)record as zero time-point. - incubated at shaked (E-coli LB borth) incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Add 50 ul of ampicillin to the Take 3 ml mixture to No ampicilin analyse O0600 the mixture broth The mixture is incubated incubator at 37c 200rpm Withdraw 3ml mixture to analyse (OD600)record as zero time-point. then Measure every 15 mins. Stop until 75 mins at shaked

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter3: The Microbiology Laboratory

Section: Chapter Questions

Problem 1TBP

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A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
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