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PCR uses a very special DNA polymerase, tell me where it came from and why it is special to PCR.
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- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymeraseDNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNases
- Drawback of PCRCan you please check my answer and make sure it is correct. Question: Describe the two roles primers play in PCR. Answer: Primers are short sequences of single stranded DNA that mark both the ends of the target sequence to be amplified during PCR. One primer attaches to the top strand at one end (forward primer) and the other one attaches to the bottom strand at the other end of the segment (reverse primer). They are made specifically to attach to these sections of DNA because the nucleotides of the primers are complementary to the target sequence. Other than marking the starting and end points for PCR, primers are also necessary for the process because without them, DNA polymerase wouldn’t be able to synthesize the complementary strand of DNA because it can only add nucleotides to an existing piece.Method for using PCR to amplify unknown sequences using known sequences by circularizing the template molecule. Reverse Transcriptase PCR Inverse PCR O Hairpin PCR Primer Structures Random Hexamers
- You are studying the DNA binding protein CLAMP and you want to determine its binding affinity for different DNA constructs, what technique should you use? ELISA PCR Chromatography Western Blotting Fluorescence polarizationBriefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysisDescribe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In simple terms so, that I can understand. Thank you
- Pcr primers are: single strand 15-25 bases long incorporated into the newly synthesized DNA strand none above all aboveWhy PCR needs the following: DNA template Primers DNA polymerase enzymes dNTPs Buffer Mg2+Which of the following polymerases should be BEST used if you would want a highly accurate PCR product? high fidelity polymerase hot start polymerase o long amplification polymerase Taq polymerase