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- Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the experiment you did in thin layer chromatography of plant pigments? How do you say so?In the context of flow cytometry data: The Lactobacillus cells also show a clear increase in PerCP (red) fluorescence when stained with SYBRGreen (SG) compared to unstained cells. Why might this be the case? Think about the emission spectrum of chlorophyll and SG in combination with the dichroic mirrors (e.g. the ‘filters’ for the detectors).Line A (entire thick black line on figure) is the absorption spectrum of the most common pigment in a newly discovered plant. What would be the predominant color of the plant ? *note the thinner blue and red lines depict other pigments, chlorophyll a and b, not being asked about in this question) 700 450 Wavelength (nm) Visible Spectrum O Yellow O White O Black O Green red Absorbance
- Briefly describe the pigments used in photosynthesis, where these pigments are located in the plant, and what these pigments do. Define both absorption spectrum and action spectrum, and describe the purpose of using a spectrophotometer to determine the absorption spectrum and how that correlates to the action spectrum. Be sure to discuss how the spectrophotometer allows the identification of the pigment solutions.In a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 Explain the reason for the differences in pH between the stroma and the thylakoid lumen.The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if needed
- 100- 50- 400 450 500 550 600 650 700 Wavelength (nm) In the absorption spectrum shown above, what wavelength ranges are not efficiently absorbed by this photopigment? 400-450nm 450-700nm 450-650nm 650-700nmin food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45At your work station, you have a spectrophotometer set at 600 nm to measure DCIP absorbance and four (4) test tubes containing different solutions. One of the test tubes is labelled “0” to blank/zero the spectrophotometer. The other three (3) test tubes contain a reaction mixture from a Hill reaction experiment using isolated chloroplasts and DCIP as an electron acceptor. DCIP is present in the oxidized state and exhibits a blue color with an absorbance at 600 nm. Upon receiving the electrons, DCIP is reduced to form DCIP-H2 that is colorless. These three (3) tubes, labelled “A”, “B”, and “C” were exposed to different light conditions during the Hill reaction. The Light conditions were random, including No light, Red light, and Green light. Please note that chlorophyll preferentially absorbs the light with wavelength of 660 nm (red) when compared to that of 520 nm (green). Your mission is to determine which Tube came from which experimental light condition. Tube: Tube…
- Define phototransduction.Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Fully excluded Partially included Fully included the activity of lysozyme was determined by looking at the increase in light scattering per unit time increase in absorbance per unit time. decrease in absorbance per unit time. increase in concentration per unit time.In etanol determination by specrophotometry, explain the purpose of the negative controls included in a protocol, what do they control for? (What will you learn if the negative control gives an unexpected result. i.e. returns a positive result?)