List all the amino acids you would expect to bind to a cation-exchange column at pH 2.
Q: Which of the following best describes how different cell types in the human body can have such…
A: DNA i.e., Deoxyribonucleic acid, is the fundamental molecule that carries the genetic information…
Q: Based on the image below, select the correct statements. Note: There may be more than 1 correct…
A: Purines are a heterocyclic organic compound that has 5-member rings fused to a 6-member ring.…
Q: Draw the biosynthetic pathway for tyrosine (anabolism)
A: Tyrosine is an polar amino acid with a hydroxyphenol side group. Tyrosine is a conditional…
Q: A protein was completely digested with trypsin and an internal peptide (i.e. not derived from the N-…
A: Amino Acid analysis or Protein Sequencing is a common method used to determine the sequence of amino…
Q: Create a diagram showing the interrelationship of Carbs-Lipids metabolism
A: The cells have various biomolecules, which play an important role. Some of the biomolecules of the…
Q: Which of the following are mechanisms that prevent the liver from using up glucose that is more…
A: Glucose homeostasis is the process of maintaining a steady and constant level of glucose in the…
Q: Can this standard curve be used for E. coli cells (why/why not?)
A: In biochemistry, a standard curve is prepared by plotting the known amounts/concentration of…
Q: "Five prime to three prime" description of a DNA strand refers to: A. the phosphodiester backbone…
A: "Five prime to three prime" description of a DNA strand refers to: B. the phosphorylated 5' carbon…
Q: For the chloroplast, the thylakoid membrane separates the thylakoid lumen from the stroma; the…
A: In chloroplasts, the movement of electrons though the electron carriers in the thylakoid membrane…
Q: 2. What is the relationship between the energy charge of a cell and control of the ATP-generating…
A: The energy charge of a cell is the ratio of high-energy phosphates (ATP and GTP) to low-energy…
Q: 7.16 Calculate the molar mass for each of the following: a. FeSO4, iron supplement b. Al2O3,…
A: The molar mass is the mass of a given chemical element or chemical compound (g) divided by the…
Q: 1.0 Y 0.5 A B T I I I I
A: Answer. The Kd ( dissociation constant) is determined through the graph between Y and the free…
Q: 11. For a reaction A + B → P +Q, which is catalyzed by an enzyme (E), the following observations are…
A: A)First understand both the mechanismPing-pong mechanism- In this type of reaction proceeds through…
Q: Describe the difference between ionic and covalent bonds.
A: Ionic and covalent bonds hold atoms in compounds. Ionic bonds form when atoms share electrons,…
Q: What are 5 regulation of carbohydrate metabolism in the liver
A: The liver controls carbohydrate metabolism and glucose homeostasis. Understanding liver carbohydrate…
Q: a. Use three-letter amino acid abbreviations to write the representation for the tripeptide shown…
A: The four types of biological macromolecules are proteins, nucleic acids, lipids and carbohydrates.…
Q: 14. Under normal circumstances: A. Adult hemoglobin (Hb) binds to oxygen more tightly than myoglobin…
A: Hemoglobin and Myoglobin are two proteins that bind oxygen. Hemoglobin binds oxygen in the lungs and…
Q: Drag and drop the statements to the correct box: Enzymes DO: Decrease required activation energy…
A: Proteins that function as catalysts in biological reactions are known as enzymes. They function by…
Q: Which of the following statements is true? a. Plotting the rate of facilitated transport against…
A: Facilitated transport, also known as facilitated diffusion, is a mechanism of passive transport that…
Q: What is the effect of incubating a mixture of RNA and DNA with 0.1 M NaOH? A. DNA is hydrolyzed to…
A: Two different forms of nucleic acids, DNA and RNA, are essential for the storage and expression of…
Q: Design a peptide sequence that will likely fold into an a-helix and form a homo- dimer (bind to…
A: The DNA in the cell has the information in the form of a nucleotide sequence which codes for the…
Q: What are the differences between miRNA and siRNA? (Select all that apply.) OmiRNAs carry the genetic…
A:
Q: DNA is highly condensed in the chromosomes. What is the sequence of events that enables the…
A: Transcription of a gene refers to production of mRNA using DNA as a template and later using this…
Q: rate of transport Vmax 1/2Vmax transporter-me ted diffusion Km simple diffusion concentration of…
A: Diffusion across a membrane is the process by which a substance moves from a region of higher…
Q: 2. Discuss an enzyme/enzymes that can use different mechanisms action depending environment.…
A: When an enzyme catalyzes a reaction that involves two substrates and after catalysis, there are two…
Q: 4. If the range for your control is 5.2-6.8 mmol/L is your control acceptable?
A: Glucose is a monosaccharide and is mainly all the complex carbohydrates that break down into glucose…
Q: Identify the nitrogen-containing base, sugar and nucleotide name for each of the following item. AMP…
A: Nucleotides are the building blocks of nucleic acids. Nucleic acids are biomolecules responsible for…
Q: Which of these fatty acids has two cis double bonds in its structure? a. Linoleic acid b. Stearic…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbon to 36 carbons. Fatty…
Q: ATP NADPH Sugar Normal Sea Water 5.2 mM 5.3 MM 6.0 mM GBR Sea Water 0.1 MM ↓ 0.1 mM 0.2 mM Calvin…
A: GBR or Great Barrier Reef is the largest coral reef, which includes marine organisms and corals. The…
Q: During glycolysis, glucose is phosphorylated and then an isomerization reaction produces fructose…
A: Step 1Glucose is a 6-carbon molecule. During glycolysis, glucose is phosphorylated and then an…
Q: 40. What would you expect about the formation of an alpha-helix for a segment of a protein chain…
A: Two types of secondary structures are abundant in protein: alpha helix and beta sheets. The alpha…
Q: Which of the following fatty acids is most likely to be a solid at room temperature? OA) B) C) D) OH…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbon to 36 carbons. The…
Q: The Lineweaver-Burk plot and other linear transformation of the Michaelis-Menten curve of kinetics…
A: Both Lineweaver-Burk and Michaelis Menten plots help in determining the parameters describing about…
Q: 1. Which is false about metabolic regulation? A: reactions with a large negative delta G are likely…
A: Metabolic regulation is the control and coordination of metabolic pathways and processes within an…
Q: H3C CH3 1. After the whole fatty acid molecule is completely broken down, how many molecules of…
A: Beta oxidation is the breakdown of fatty acids into acetyl CoA and other products based on carbon…
Q: ou are given a drink that has been sweetened with either maltose or sucrose. Using the following…
A: The Somogyi-Nelson test is a quantitative test used for the estimation of reducing sugar in the…
Q: How many total ATP are REQUIRED to make 2 molecules of glucose in the liver via GNG starting with 4…
A: Gluconeogenesis (abbreviated as GNG) is the metabolic pathway that synthesizes glucose molecules…
Q: Select the statement that is NOT correct O Most plasma lipoproteins are synthesised in the liver The…
A: Metabolism refers to the chemical processes that occur within living organisms to maintain life. It…
Q: What is the energy yield in ATP molecules associated with pyruvate ? acetyl-CoA + CO2
A: The process by which pyruvate is converted into acetyl-CoA and carbon dioxide (CO2) is known as…
Q: From the graphs given, can you find the Km from the Michaelis-Menten curve and the Lineweaver-Burke…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: What is the term transamination means in amino acid metabolism? Provide examples; how does it differ…
A: This answer defines "transamination" in amino acid metabolism and contrasts it with oxidative…
Q: The hydrolysis of glycogen to yield glucose is catalyzed by the enzyme phosphorylase. Caffeine,…
A: Glycogen phosphorylase is an enzyme that catalyzes the breakdown of glycogen into…
Q: A 32-year-old woman was admitted to the hospital following 2½ days of severe vomiting. Before this…
A: The maintenance of the body's numerous physiological processes depends heavily on minerals. They are…
Q: THINK Answer this question individually 1. Why does the fermentation of sugars, such as glucose and…
A: The cell undergoes cellular respiration and produces energy by using organic molecules like glucose…
Q: 1.When the side-chain of a Asp in a protein is in close proximity to the side-chain of an Arg at…
A: Amino acids are biomolecules that have a carboxyl group, an amino group, a hydrogen atom and a…
Q: 17. Melittin is a 26-amino acid polypeptide found in bee venom. In its monomeric form, melittin is…
A: As per the fluid mosaic model, biological membranes are made up lipid bilayer with proteins…
Q: 15.76 Because peanut oil floats on the top of peanut butter, the peanut oil in many brands of peanut…
A: Triglycerides or triacylglycerides or triglycerols are fatty acid esters of glycerols. In…
Q: Can all abnormal hemoglobin be diagnosed by electrophoresis? Explain your answer in detail.
A: Electrophoresis means the migration of charged particles under the influence of an electric…
Q: Why is yeast used as a source of RNA? Explain in 1-3 sentences
A: Yeasts have around 6% of total biomass as RNA, this is the highest amount of RNA content among all…
Q: Given that the enzyme pyruvate carboxylase converts Pyruvate into Oxaloacetate and this is an…
A: A metabolic pathway's precursor or intermediate molecule is replenished by an anapleurotic reaction.…
List all the amino acids you would expect to bind to a cation-exchange column at pH 2.
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Given the amino acids below, what amino acid will elute first in a cation-exchange column. 1. Aspartate or Lysine using a buffer at pH 7 2. Arginine or Methionine using a buffer at pH 7 3. Lysine or Valine using a buffer at pH 7 4. Glycine or Valine using a buffer at pH 7 5. Serine or Alanine using a buffer at pH 7Vasopressin is a peptide anti-diuretic hormone that is important for maintaining serum osmolality. Vasopressin contains 9 amino acids in the sequence shown below. How many peptide bonds would be cleaved when vasopressin is treated with chymotrypsin? Chymotrypsin is an endopeptidase that hydrolyzes on the C-side of phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp). Vasopressin amino acid sequence: Cys - Tyr - Phe - Gln - Asn - Cys - Pro - Arg - Gly O peptide bonds cleaved - no reaction with chymotrypsin O 1 peptide bond cleaved O 2 peptide bonds cleaved 3 peptide bonds cleavedConsider a hexapeptide of the sequence thr asn glu trp lys gln. After complete hydrolysis, which amino acid would elute first from a cation exchange column at pH 7? O Asn O GIn Thr Glu O Trp O Lys
- A tripeptide has the following sequence: Glu-Trp-Arg What is the net charge of this tripeptide at physiological pH?Above or below what pH would the polypeptide become fully mobile on a cation exchange column? Explain reasoning please. Per my understanding, I believe I need to find pI and then select a pHThe order of elution of amino acids X, Y, VW and Z in a cation exchanger eluted with a buffer of increasing pH is as follows: Z, W, Y, X (last). If the amino acids are glutamic acid, arginine, phenylalanine and alanine, what amino acid can Z be? O A O F O E OR
- Place each charge form of alanine under the pH condition where it would be the predominant form. The pK₁ values for the carboxyl group and amino group of alanine are approximately 2.3 and 9.7, respectively. pH 11 H | H₂C-C-COO NH₂ Does not occur in significant amounts at any pHA mixture of Alanine (pl 6.02), Glutamic Acid (pl 3.22), Glycine (pl 5.79), Lysine (pl 9.74) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2 ? v First 1. Alanine v Second 2. Glutamic Acid v Third 3. Glycine v Fourth 4. Lysine v Fifth 5. Threonine 6. No separationEach ionizable group of an amino acid can exist in one of two states, charged or neutral. The electric charge on the functional group is determined by the relationship between its pKa and the pH of the solution. This relationship is described by the Henderson-Hasselbalch equation. a Histidine has three ionizable functional groups. Complete the equilibrium equations by assigning the proper pK₁ for each ionization and the net charge on the histidine molecule in each ionization state. H || H₂N-C-C-OH | CH₂ 2 + HN -1 -NH pKa = 1.8 +2 || H₂N-C-C-0- 1 CH₂ + HN -NH pKa = 9.17 Answer Bank pK₂ = 7.59 H|| H₂N-C-C-0- | CH₂ N pKa -NH = 6.0 -2 н II H₂N-C-C-0- | CH₂ +1 N C -NH 0
- An oligopeptide has the following amino acid sequence: NH2-Ala-Glu–Leu–Trp–Tyr-Ser–Gly–Lys–Leu-Ala–Arg-Ala-Phe-Ile-Pro–Gly-COOH a) Estimate the net electric charge of the molecule at pH 8.0 and pH 11.0. b) If the above peptide is passed through a cation exchange chromatographic column (that is, the matrix of the column has negative charges) stabilized at pH 8.0, would you expect it to be retained on the column? c) Indicates the number of fragments, and the sequence of each of them, that would be obtained when treating the peptide in question with: i) trypsin, ii) chymotrypsin.Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the…The OH group of the serine side chain (R-OH) can undergo dissociation to form R-O- and H+. However, the ratio of the protonated to unprotonated forms of this group at pH 7 is approximately 1,000,000 to 1. Calculate the pKa of the serine OH group.