Given the amino acids below, what amino acid will elute first in a cation-exchange column.1. Aspartate or Lysine using a buffer at pH 72. Arginine or Methionine using a buffer at pH 73. Lysine or Valine using a buffer at pH 74. Glycine or Valine using a buffer at pH 75. Serine or Alanine using a buffer at pH 7

Cation exchange chromatography involves the exchange of the positive cations present in the solution…

Answered: Given the amino acids below, what amino…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

What is the percent ionization of these amino acids? 1. Aspartame at pH 5.4 2. Glutamate at pH 11.8 3. Histidine at pH 8.0 4. Tyrosine at pH 8.0
Vasopressin is a peptide anti-diuretic hormone that is important for maintaining serum osmolality. Vasopressin contains 9 amino acids in the sequence shown below. How many peptide bonds would be cleaved when vasopressin is treated with chymotrypsin? Chymotrypsin is an endopeptidase that hydrolyzes on the C-side of phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp). Vasopressin amino acid sequence: Cys - Tyr - Phe - Gln - Asn - Cys - Pro - Arg - Gly O peptide bonds cleaved - no reaction with chymotrypsin O 1 peptide bond cleaved O 2 peptide bonds cleaved 3 peptide bonds cleaved
In a solution with pH ~7 most of the amino acids are form zwitterions. Name the amino acids which are charged positively and negatively and draw them in ionized forms.
Three proteins with an overall charges of 0, -5, and -8 are passed through an anion exchange column equilibrated in a buffer with low ionic strength and eluted with an increasing gradient of salt in a buffer. Which protein will elute last from the column? 1. Protein with no charge. 2. Protein with eight negative charges. 3. Protein with five negative charges. 4. Both charged proteins will elute at the same time. O 2 O 4 O 3 O 1
The pH vs charge graph for a triprotic amino acid is shown below. Please answer the following questions about the amino acid. 2 1.5 1 0.5 -0.5 -1 4 8 10 12 14 pH a. Which of the following triprotic amino acids is this? Histidine b. What is the isoelectric point? (Please select the appropriate range in which the isoelectric point falls) (Hint - look up the pka values in the pKa table for amino acids, which was in video one of today's lecture) 7-8 c. What form of this amino acid dominates at a pH of 8? Please select the correct form for each jonizable group. Alpha amino NH3+ Alpha carboxyl СОО- R| NH Net Charge
Draw a diagram of a Ramachandran plot. Indicate the location on the plot of an amino acid residue with phi = - 90 degrees and psi = + 120 degrees.
Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.
which of the following statements is correct regarding the affect of pH on the ionization of R groups in amino acids. Put yes or no next to each number. 1) at an acidic pH, amino groups in an R group tend to lonize by accepting a proton and have a positive charge2) at an acidic pH, carboxyl groups in an R group are likely to be in an unionized state.3) at an alkaline pH, amino groups in an R group have a tendency to remain unionized (and uncharged).4) at an alkaline pH, carboxyl groups in an R group have a tendency to lonize by loosing a hydrogen ion (H+)5) R groups that do not have an amino or carboxyl group do not ionize at pH of the environment changes.
1) If the pH of the rain drops from 6 to 5 due to air pollution, the rain has become: a) two time more acidic b) four-time more acidic c) ten time more acidic d) one thousand times more acidic 2) which of combination of statement is correct ? the denaturation of protein 1- causes changes in tertiary structure 2- is always an irreversible process 3-has no influence on the solubility 4-occurs only by interaction with heavy metals 5-does not influence covalent bonds and hydrogen bonds select one: a) only 1 is correct b) only 1 and 2 are correct c) only 3 and 4 are correct d) only 3, 4 and 5 are correct e) only 1, 2 and 5 are correct
List all the amino acids you would expect to bind to a cation-exchange column at pH 2.
Draw a graph using optical densities of the amino acid ninhydrin complexes at different wavelengths
For the amino acid tryptophan: Draw its complete protonic equilibria. Indicate the net charge of each form and encircle the zwitterionic form. Provide labels (A, B, C, etc.) for each form. b. Calculate the IpH of the amino acid. c. At what pH range(s) it can act as a buffer? d. What will be the predominant form(s) of the amino acid at pH (i) 1.5; (ii) 3.0; (iii) 4.5; (iv) 6.0; (v) 7.5 and (vi) 9.0?

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