In a 250ul reaction containing 0.3 nmol of a Michaelis enzyme with Km = 4.4 x 10 M and saturating substrate concentration, product was formed at an initial velocity of 6.3 x 10³ M min-1. What is kcat for this enzyme? Give your answer in 3 sigfigs in scientific notation, for example 1.20 e
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- Question 16 For a Michaelis Menten reaction the following rate constants were observed: k1 = 7x 107/M-sec k1 =1x 103/sec k2 = 2 x 104/sec The value of Km is: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 0.3 M b 0.03 M 0.003 M d. 0.0003M e 0.00003 M f 0.000003 M 0.0001 M 0.01 M i 0.001MQUESTION 35 Calculate the reaction rate of catalase at room temperature using the following data. Depth of Hydrogen Peroxide Solution Time = 66.3 seconds 2.2 cm O 3.000 68.50 0.080 O 30.140 O 0.033QUESTION 3 In the presence of 0.000005 M competitive inhibitor, an enzyme exhibits an apparent Km of 0.002M. If the Kt of the inhibitor is 0.0000003 M, what is the true Km of the enzyme? Give your answer with 3 significant figures an scientific notation. For example, 0.000023434 is 2.34e QUESTION 4 Approximately how many domains are in an 82.5 kDa protein? O a. 2 O b.3 O C. 4 O d. 1 2.5 QUESTION 5 How long is an a-helix that contains 36 amino acid residues? 6.0 nm 5.4 nm 78 Mostly clody a n Click Save and Submit to save and submit. Click Save All Answers to save all ansuers P Type here to search
- QUESTION 2An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units perminute. Given that the molar absorption coefficient (E) is 6220 M-1 cm-1 and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in umol perminute per mL?Question 4 If 32p – labeled (radioactive phosphate) inorganic phosphate were introduced to erythrocytes undergoing glycolysis which of the following molecules would you expect to detect 32 p in Glycolytic intermediates? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a Fructose 1-6 bisphosphate b 3-Phosphoglyceraldehyde Dihydroxyacetone phosphate d 2-Phosphoglycerate e 1-3 bisphosphoglycerate f Phosphoenolpyruvate 3-Phosphoglycerate h Pyruvate i None of the aboveQuestion 9: For the reaction catalyzed by the enzyme hexokinase, Glucose + ATP = glucose 6-phosphate + ADP, the equilibrium constant is 7.8 x 10² at 298 K. Please answer the following independent questions. A. Determine the standard Gibbs free energy of the reaction. B. In dying E. coli cells, the concentrations of metabolites were found to be: [ATP] = 0.5 mM, [ADP] = 100 mM, [glucose] =0.1 mM, and [glucose 6-phosphate] = 2 mM ay 298 K. Does the reaction proceed spontaneously? C. What would be the ATP:ADP ratio at equilibrium at 37 C if the concentration of glucose is 100- fold less than that of glucose-6-phosphate? The standard enthalpy of this reaction at 298 K is - 23.8 kJ/mol.
- QUESTION 2 CH3COO-C₂H5 + H₂O → CH3COOH + C₂H5OH Ethyl acetate Acetate Ethanol Ethyl acetate, acetate and ethanol were added to water solution in concentrations of 0.3M and left at 25°C until the solution reached an equilibrium state: ethyl acetate = 0.276 M; acetate=0.012 M; ethanol = 0.012 M; The experiment was repeated at the same temperature with the initial concentration of 0.1M of ethyl acetate. What will be the concentration of ethyl acetate (in moles/liter) be once equilibrium is achieved?Question 33 Orciprenaline (C₁₁H₁7NO3, 211.258 g/mol) is a bronchodilator used in the treatment of asthma. The BP assay for orciprenaline tablets is described below. HO The following information was obtained during the assay: Weight of 20 tablets = 2.5564 g Weight of tablet powder assayed = 0.5266 g Absorbance reading - 0.5868 Stated content per tablet = 20 mg A (1%, 1 cm) at 276 nm = 72.3 OH H N OH "Weigh and powder 20 tablets. Shake a quantity of the powder containing 80 mg of orciprenaline sulphate with 50 mL of 0.01 M hydrochloric acid (HCI), filter and add sufficient 0.01 M HCI to the filtrate to produce 100 mL. Dilute 10 mL to 100 mL with 0.01 M HCI and measure the absorbance of the resulting solution at the maximum at 276 nm." Orciprenaline 4 Answer the following questions, explaining your reasoning clearly. a) Calculate the amount of orciprenaline in the tablet powder analyzed. b) Calculate the average amount of orciprenaline per tablet. c) Calculate the percentage of the stated…QUESTION 58 The following results were obtained from a chromatography experiment on spinach extract. Calculate the rata of miprmo (R ) for chlorophyll A. Chlorophyll A = 13 Chlorophyll B 8 Xanthophyll 20 Carotene =65 Solvent front = 68 O 0.12 O 0.19 1.00 O 0.29 O 0.96 ck Save and Submit to şave and submit. Click Save All Answers to save all answers.
- Question:- The enzyme aromatase is found in the cytoplasm of some cells and converts testosterone to estrogen. You decide to test aromatase from a particular cell, and oops, your lab partner admits he drastically increased the pH in all the test tubes. Which of the following is a likely result? a. The enzyme will be denatured and the substrate will not bind to the active site. b. The enzyme will convert testosterone to estrogen at a faster rate. c. The mistake will have no effect on the experiment, because enzymes are not sensitive to pH. d. The free energy will be lowered and the reaction will not proceed spontaneously.QUESTION 3 Biocatalysts are effective because: O a. They increase the activation energy of a reaction Ob. They stabilize the transition state O. They decrease the rate of the reaction Od. They decrease the activation energy of a reaction QUESTION 4 Which of the following statements is INCORRECT regarding L-amino acids and D-amino acids? O NH3 group on the left of the chiral carbon in both isomers O b. They are nonsuperimposable mirror images of each other O NH group on the left of chiral carbon in L isomers Od. They are enantiomersQUESTION 10 Select a property that does not belong to allosteric enzymes. They conform to hyperbolic Michaelis-Menten kinetics They may have binding sites for regulatory molecules that are separate from active sites They tend to have a signmoidal curve of rate versus [S] They undergo conformation changes as a result of modulator binding.