Aldolase enzymes have the following rate constants. k₁ = 10³M ¹s¹, k-₁ = 4.4x10 s ¹, k₂ = 10's-¹ -1 Enzyme concentration is 10 6 M and substrate concentration is 10-³M (a) Use the assumption of Michaelis and Menten to find the rate of enzyme reaction (b) As a substance (1) was added, the enzyme reaction was reversibly inhibited, and the lineweaver-Burk plot changed as follows. When [1]=2KI, find the reaction rate. 1/v [0]=0 1/[S]

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Aldolase enzymes have the following rate constants.
-1
k₁ = 10 M¹s¹, k-₁ = 4.4×10's-¹, k₂ = 10's-¹
S
Enzyme concentration is 106M and substrate
concentration is 10-³M
(a) Use the assumption of Michaelis and Menten to find
the rate of enzyme reaction
(b) As a substance (1) was added, the enzyme reaction
was reversibly inhibited, and the lineweaver-Burk plot
changed as follows. When [1]=2KI, find the reaction
rate.
1/v
(1)=0
1/[S]
Transcribed Image Text:Aldolase enzymes have the following rate constants. -1 k₁ = 10 M¹s¹, k-₁ = 4.4×10's-¹, k₂ = 10's-¹ S Enzyme concentration is 106M and substrate concentration is 10-³M (a) Use the assumption of Michaelis and Menten to find the rate of enzyme reaction (b) As a substance (1) was added, the enzyme reaction was reversibly inhibited, and the lineweaver-Burk plot changed as follows. When [1]=2KI, find the reaction rate. 1/v (1)=0 1/[S]
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