II. EVACUATED TUBES, ANTICOAGULANTS, AND LABORATORY USES Complete the following table Additive/Anticoagulant Sodium Citrate Function Laboratory Test(s) Нерarin KŻEDTA Gel Separator
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- 1) Determine the MICS on the simulated microbroth-dilution results from an MIC Assay on Staphylococcus aureus. Legends: PC: Positive Control (No antimicrobial agent) NC: Negative Control (No bacteria) No Growth With Growth µg/mL: 128 64 32 16 8 4 2 1 0.5 0.25 PC NC 2 3 4 5 7 8 9 10 11 12 Amoxicillin A Ticarcillin Piperacillin C D Cefotaxime E Ertapenem F Nalidixic Acid G Ciprofloxacin H Gentamicin а. Fill out the MICS on the table below. MIC Susceptibility to Staphylococcus aureus Antimicrobial Agent (Hg/mL) (Susceptible, Intermediate, or Resistant) Amoxicillin Ticarcillin Piperacillin Cefotaxime Ertapenem Nalidixic Acid Ciprofloxacin Gentamicin B6 2 4 3 5 LABORATORY REPORT FORM EXERCISE 25 ASSAY OF ANTIMICROBIAL AGENTS: DISK-DIFFUSION METHODS What is the purpose of this exercise? Evaluate 1. For each of the disinfectants or antiseptics tested, determine the active ingredient. What class of inhibitory agent does it belong to and what is its mode of action? CHEMICAL AGENT Lysol Sanitizer Foaming Handwash Detergent. Desinfecting Hand Sanitizer ACTIVE INGREDIENT(S) Alhyl Dinethylaenzy/a. chloroxylenol 0.3% Alkyl, Dimethyl benzyl ammonium chlorides ethy benzyl A. C. Ethyl Alcohol 70% 1/Cleansing s Pray Hydrogen Peroxide Benzalkonium Lidocaine HC) An Septic CLASS OF AGENT MODE OF ACTION1) Order i Ampicillin 250mg IVPB qsh. Add to 5omL of Dsul and infuse over 20 minutes. Supply : Ampicillin 1g vial Reconstitutionn Instructions:add 9.5mL 0f Sterile for a Cuncentratiun of 19/1omL. water How mL of the reconstituted medication will you mary add to the minibag? anial N set MA with a drop factor of 15glm using what is the Infusiom rate ! a m If an electronic pump is auaitable,uhat is the infusiir rate in mL/hr?
- Order: metoclopramide 5 mg PO Q6HPatient 30 kg; 3 years oldSupply: 5 mg/5 mLLiterature: 0.1 to 0.2 mg/kg/dose up to four times per day Order: cefotaxime (Claraforan) 0.5 g IM Q6HPatient: 48 lbSupply: 300 mg/ reconstituted solutionLiterature: 100 to 200 mg/kg/day divided Q6HLung Cancer Patient Whats the use of the given medications below: ● Cisplatin100 mg/m2 IV q4 weeks● Trametinib Dimethyl Sulfoxide 2mg/tab 1 tablet OD● Roflumilast 500 mcg PO● Spiriva Respimat: 5 mcg (2 actuations; 2.5 mcg/actuation)● Budesonide 1 neb q6hr● Hydrochlorothiazide 25 mg tab PO OD● Levothyroxine 175 mcg PO OD● Hydralazine 50 mg tab PO TID● Metformin 500 mg tab PO BIDAnalysis of the pathological constituents GIVE THE FOLLOWING: a. Name of Test/Test Reagent b. Positive Result Blood (Note: Presence of blood in urine is termed as hemolobinuria) To 1 ml of freshly prepared Benzidine solution (sat’d sol’n of Benzidine in glacial Acetic acid) and 10 drops of urine sample. Add 5 drops of 3% H2O2. A positive result is indicated by a blue green color.
- Lung Cancer Patient Whats the use of the given medications below: ● Nebivolol 5 mg tab PO OD● Aspirin 81 mg tab PO OD● Vitamin D3 1000 units capgel PO OD● Clopidogrel 75 mg tab PO OD● Isosorbide mononitrate 60 mg PO OD● Rosuvastatin 40 mg PO OD.● 300 mg Carboplatin was injected intraperitoneally● Kabiven (1440mL) infusion at 60cc/hr● Omeprazole drip of 80mg incorporated in PNSS 100mLAnswer the following questions: Therapeutic Classification of Sweet Orange Peel Tincture, USP Indication of Sweet Orange Peel Tincture, USPDose ordered garamycin 60 mg Im stock available gentamicin injection 40 mg/ml (20-ml vial) how many ml are need for the dose ordered?
- Discuss the procedure and application for the agar well diffusion method of evaluating antimicrobial activity.how to prepare these dilutions? "Using a normal saline, prepare dilutions of the diphtheria toxoid standard, the dilutions form a series differing by not more than 2.5-fold steps and in which the intermediate dilutions." Needs 3 dilutions, volume on each dilution = 4.5mlstandard concentration = 213IU , dilute not less than 30IU.Draw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.