If acyetal-CoA dehydrogenase was unavailable, malonyl-CoA could not be made from aceytal-CoA. That would yield a glut of acetyl-CoA that could not be shuttled into fatty acid synthesis. Would acetyl-CoA then be preferentially be taken up in the TCA cycle? Beta-oxidation? or another pathway?
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- Complete oxidation of a 16-carbon fatty acid can yield 129 molecules of ATP Study Figure 19.2 and determine how many ATP molecules would be generated if a 16-carbon fatly acid were metabolized solely by the TCA cycle, in the form of S acetyl-CoA molecules.Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.The large amount of energy used during aerobic exercise(e.g., running) requires large amounts of oxaloacetate.Explain why acetyl-CoA cannot be used to produceoxaloacetate in this circumstance. What is the sourceof oxaloacetate molecules during aerobic activity?
- Name one reaction of glycolysis, one anaplerotic reaction, and one reaction of TCA cycle that acetyl-COA allostericqally regulates ( and declare whether it is an activator or inhibitor.)Step 2 of B-oxidation involves a reaction with chemistry characterized as a dehydration. B-Oxidation is shown below. H HO || || C-C-S – CoA FAD но R-CH2- C Acyl-CoA dehydrogenase H-C-C -S- CoA Acetyl-CoA Fatty acyl-CoA H FADH2 - CH2 - -c-S- CoA R- Thiolase Fatty acyl-CoA shortened by two carbons COA-SH H. он Successive cycles R-CH2-C-C- -C-S- CoA R– CH2 C=C-C-S-CoA H B-Ketoacyl-Co.A trans-A²Enoyl-CoA NADH + H* H,0 H H L-Hydroxyacyl-CoA dehydrogenase Enoyl-CoA hydratase R- CH,-c-c-C- S-CoA Но H NAD+ L-B-Hydroxyacyl-CoA True O FalseBefore β-oxidation, fatty acids must be converted to their CoASH derivatives. Explain why this reaction is necessary.
- Another biochemical study from another patient reveals a 100X increase in the KM value for the enzyme succinyl-CoA synthetase for this individual (only isozyme expressed). What metabolic and physiological consequences would you predict resulting from this enzymatic change?1) Humans do not undergo net synthesis of carbohydrate from acetyl- CoA, yet carbons of acetyl -CoA can be incorporated into glucose and amino acids. Show pathways as to how this can happen. 2) A molecule -X inhibits oxidation of stearoyl-CoA by isolated mitochondria but has no effect on palmitoyl-carnitine oxidation, explain.The total degradation of a fatty acid with an odd number of carbons yields acetyl-CoA and another compound, X. Show the structure of X, and describe the pathway by which it is converted into a citric acid cycle intermediate, including where any cofactors participate.
- Cholesterol synthesis and ketogenesis share the first few reactions after which the pathways deviate. Provide a schematic presentation showing the production of ketone bodies from acetyl-CoA and where cholesterol synthesis deviates from this pathway (first intermediate that differs between the two pathways).Fatty acids are oxidized by acyl COA dehydrogenase. Branched fatty acids, containing a methyl group at beta carbons cannot be oxidized by this enzyme. How does the oxidation of such branched fatty acids take place? Explain with all set of reactions and enzymes involved.. give necessary detail.. avoid cpy rightingThe acyl-CoA dehydrogenase deficiency diseases are a groupof inherited defects that impair the b-oxidation of fatty acids.Symptoms of the disease range from nausea and vomiting tofrequent comas. Symptoms may be alleviated by eating regularly and avoiding periods of starvation (12 hours or more).Why does this simple procedure alleviate the symptoms?