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If a spectrophotometer is available, dispense the culture into labeled corvette and determine the optical density of each culture
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- True or false A spectrophotometer is used to directly count the number of cells in a liquid culture media.The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope at 10x magnification. Samples were stained with Lugols solution for better viewing. Differentiate these two imageThe first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stain
- Write an introduction related to culture media and isolation of pure culture technique (streak plate and spread plate method)What advantage does the oil immersion objective have over the low power objective when doing bacterial examination?Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…
- Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the Gram staining procedureWhat are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank youWhy is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
- if an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400x