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if an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to be in fading color and the shape of the specimen seems to be diffused. How are you going to troubleshoot the focusing of the specimen for you to identify clearly the bacteria?In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?
- (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
- You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.List the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.
- Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents? a)Grama's staining procedure b)simple staining procedure c)Acid -fast staining procedure d)Non -acid fast staining procedure e)Scaeffer -Fulton staining procedureAfter performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.Which step of the gram stain procedure is most critical?why?
