Humans have very similar DNA sequences, with approximately 999/1000 letters being One possible way of telling the difference between these very similar sequences is by: Genetic engineering Analyzing RFLPS using gel electrophoresis Comparing the total amount of DNA from two or more people
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- DNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of theseOrder the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of DNA to be sequenced add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase a primer binds to the single-stranded DNA template DNA polymerase extends the primer, incorporating deoxynucleotides a labeled dideoxynucleotide terminates the growing DNA chain gel electrophoresis separates the mixture of DNA fragments by size The DNA sequence is determined denature the double-stranded DNA Answer BankK What is an example of positive feedback? sweating when you are hot increased respiration during exercise decreased blood glucose levels when insulin is released maintenance of blood pH levels labour contractions during birth
- Genetic Engineering Terms Name: Draw a line to connect each pair of boxes DNA sequencing The use of an organism, or a component of an organism or other biological system, to make a product or process. DNA cloning The sequencing, analysis, and cutting-and-pasting of DNA A technique to make many copies of a specific DNA region in vitro (in a test tube rather than anorganism) Recombinant DNA Polymerase chain reaction A technique used.to separatę DNA fragments according to their size Gel electrophoresis DNA that is assembled out of fragments from multiple sources Biotechnology A molecular biology technique that makes many identical copies of a piece of DNA, such as a gene DNA technology The process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA. ww tİ6111749/enetic-engineering-temsWhich ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt rubbing alcohols detergent solution water What is the purpose of PCR? To copy and then make many copies of a specific region of DNA To copy the entire human genome To make just a few copies of DNA To reveal the sequence of a piece of DNA If enzyme catalase has an optimum pH of 7.0, What do you think will happen en the pH is lowered to 3.0? The rate of its enzyme action is increased. Its enzyme action is not affected at all. The rate of its enzyme action is decreased. Its enzyme action ceases or stops.What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic site? Sequencing of the DNA by Sanger Northern blotting Southern blotting RFLP analysis in agarose gel electrophoresis PCR
- Examine the DNA fragment sequence below. Your job is to design primers for PCR that would be able to amplify this DNA fragment. Design the primers so that they are 7 bases in length. Don’t forget to indicate direction (polarity) of the primers. Also describe where the primer would bind (i.e. top or bottom strand, left or right side of the DNA strand). Please organize your response so that each primer, and associated information, is separated by at least one blank line 5’ - TCCACTTGCTGTGTAGCTAAATCATATAACAG3’ - AGGTGAACGACACATCGATTTAGTATATTGACA cloned fragment of DNA was sequenced by using the dideoxy method. A part of the autoradiogram of the sequencing gel is represented below. Deduce the nucleotide sequence of the DNA nucleotide chain synthesized from the primer. Label the 5' and 3' ends. ddATP ddTTP ddGTP II ddCTPGel Electrophoresis is used in many different forms to learn about DNA, RNA or proteins. Research one laboratory method or technique that uses DNA electrophoresis in order to learn more or make determinations about DNA, RNA or Proteins. Name of technique or method Brief description of what the electrophoresis results are used for in the method. Include a link to your resources Include an image of the results or technique you describe
- Choose the single most appropriate description of how most Next Generation sequencing methods work. Template DNA is attached to 'chips', free di-nucleotides are added to synthesise new DNA, computers read the results. Template DNA is attached to 'chips', di-deoxy nucleotides are added to synthesise new DNA, computers read the results. Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. They are bound to a membrane and sequenced using lasers. Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. The fragments are then bound to a membrane and sequenced using light emission as the reporter. Sequencing by synthesis of short template fragments, using various 'reporters' and computing power to rebuild lengthy sequence data in silico using reference genomes. Sanger sequencing of short fragments, using various 'reporters' and computing power to…Which of the following is not true about microarrays? Group of answer choices A microarray is a glass slide with single-stranded DNA attached to it. A microarray is covered in spots, where each spot represents a different sequence that will be assessed in the experiment. A microarray can assess hundreds of thousands of variants simultaneously. The same microarray slide can be used either for DNA microarrays or RNA microarraysWhich of the following most accurately describes the process of DNA cloning? set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes set of laboratory procedures that uses living cells to mass-produce specific DNA fragments set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid