gel filtration chromotagrpahy
Q: 9. Name the following compounds using R.S designations: 'CH, a. HO H H₂ C CH₂CH, CH₂OH B. HỌ-CH,…
A: R-S system - The R / S system is an important nomenclature system for denoting enantiomers. This…
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A: Proline is one of the 20 standard amino acids which has an amino group (NH2), a carboxyl group…
Q: 9. The T conformation of the hemoglobin tetramer is stabilized (in part) by a salt linkage between…
A: Hemoglobin is a globular protein, ie it is roughly spherical. It is a tetramer of two types of…
Q: fertilizers
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Q: Choose any/all that apply to mRNA processing in eukaryotes. The "starter/initiator" methionine codon…
A: The transcript that is produced as a result of transcription of an eukaryotic DNA sequence is called…
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A: Amino acids are the building blocks of proteins and play essential roles in various biological…
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A: Enzymes catalyze reactions using various types of mechanisms. Some of the most observed mechanisms…
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A: A typical structural motif in proteins is a beta sheet, commonly referred to as a beta strand. It is…
Q: AND SHOW THE REACTION! To trap glucose in a cell, the following reaction is catalyzed by the…
A: Hexokinase is the most important rate limiting enzyme catalyzing Irreversible first reaction in the…
Q: At pH ___, the pentapeptide AEHVC would contain two positively charged groups and one negatively…
A: Recall that:Amino acid sequences are written with N-terminal amino acid on the left and C-terminal…
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A: NADH produced through glycolysis is present in the cytosol of the cell. The glycolytic pathway is…
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A: The organic building components of proteins are known as amino acids. They have a hydrogen atom, an…
Q: The phrase "dietary amino acids are absorbed during digestion" is used to describe when: Amino acids…
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A: Red blood cells (erythrocytes) have presence of a protein which is known as Hemoglobin. Hemoglobin…
Q: the resonance stabilization of the amide linkage has profound effects on protein structure. Which of…
A: Answer :- The amide linkage, a fundamental component of proteins, plays a pivotal role in…
Q: Peptide A: AFEQHSR Calculate the isoelectric point of Peptide A using the pKa values in Table 4-1 of…
A: A peptide is a short chain of amino acid residue linked via a peptide bond. Recall that: Amino acid…
Q: You run a series of assays at 25°C on enzyme A. You measure the velocity for a range of S…
A: This question can be solved using Lineweaver Burk plot. This graph also represents the enzyme…
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A: The decomposition of crystalline N2O5 N2O5(s)➡️ 2NO2(g) + 1/2 O2(g) At 25°C Standard state enthalpy…
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A: Amino acids are the monomer units of proteins. Out of the 20 general proteogenic amino acids, some…
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A: The pH is a numerical measure of the acidity or alkalinity of a solution.The range of pH scale is 0…
Q: Trypsin catalyzes the cleavage of: a) all peptide bonds b) peptide bonds after Lys or Arg c)…
A: Proteases are the enzymes that hydrolyse proteins into amino acids or peptides. They break the…
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Q: hy do we generate ketones when we have low CHO levels
A: Acetyl co enzyme A is formed in the liver during oxidation of fatty acids, may either enter the…
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A: In order to solve this problem, first we need to calculate the decay constant (). The half life…
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Q: [2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of 0.1M NaOH and…
A: A method employed in laboratories for calculating an amino acid's pKa values and isoelectric point…
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Q: The molecule below is found in 0 ORNA. HO Osteroids. Oprotein. Ostarch. ODNA. .0 N OH OH NH 0
A: From the structure you can see there is a pentose sugar along with nitrogenous base.. This kind of…
Q: membrane potential of a neuron drops to -55mV and sodium ions start to flow from outside to inside…
A: This question can be calculated by Nernst equation. This equation provides the relationship between…
Q: Draw a generic dipeptide, and write out the mechanism describing its cleavage catalyzed by a serine…
A: Serine proteases are protein cleaving enzymes that have Serine, Histidine and Aspartate (the…
Q: 3. A scientist wishes to find the sequence of a certain protein. Treatment with FDNB releases…
A: In the quest to determine the amino acid sequence of a certain protein, a series of enzymatic…
Q: The reaction coordinate diagram shown below is for the mitochondrial enzyme ATP synthase. KJ/mol ATP…
A: A reaction coordination graph is a graphical representation of the free energy changes that occur…
Q: 3.) The enthalpy change associate with a biological process is -13200 cal/mol at 298K and +14700…
A: The heat capacity of a system is the amount of heat that must be supplied to a system to raise its…
Q: From the peptide chain: Met-Tyr-Phe-Arg-Ile-Glu-Asn-Asp-Ser, draw the structure of the product…
A: There are four types of biological macromolecules: proteins, nucleic acids, lipids and…
Q: How many peptide bonds are present in the peptide shown below?
A: By removing a water molecule from an amino group of one amino acid and a carboxyl group of the…
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A: Aminoacids are classified based on the nature of their side chain. They areAcidic aminoacids :…
Q: gives the turnover number of four representative enzymes. Enzyme Ribonuclease Fumarase Lactate…
A: Turnover number of an enzyme is defined as conversion of substrate molecules per second into its…
based on the chart answer the following:
1. which proteins will be most excluding use gel filtration chromotagrpahy
2. which proteins will migrate closer to the anode using SDS- PAGE
3. which protein will have the most net positive charge at a ph of 7.4
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- The biosynthesis of vitamin D₂ is a two-step reaction, requiring two types of concerted pericyclic reactions. Draw in the arrows for each step and identify the structure of precalciferol. If an electrocyclic reaction is required, identify if it occurs through a conrotatory or disrotatory reaction. HO H H ergosterol hv precalciferol Cast HO ergocalciferolDescribe the biochemical role of coenzyme Q10 and how supplementation may impact metabolism. Be sure to address the key terms listed below as part of your discussion; you may address additional etiologies as well. coenzyme Q Complex I Complex II Complex III ubiquinone ubiquinol Electron transport chain Oxidative phosphorylation Reactive oxygen speciesThe table below shows the purification of liver lactate dehydrogenase. Purification Table of liver LDH Step Crude Extract Ammonium Sulfate DEAE-Sephadex 5'AMP-Sepharose Volume (mL) 200 100 112 40 LDH activity (Units/mL) (mg/mL) [Protein] 47.0 50.00 90.0 63.00 51.0 1.50 137.0/ 0.4 Calculate the LDH yield for the ammonium sulfate step. Provide answer to one decimal only. Show Transcribed Text this is biochemistry and the topic is protein purification could you pleaae show me the full workings even the workings for the comversions
- Consider the proteins in the table below. Protein Isoelectric point (pl) М.W. Alcohol dehydrogenase 150,000 5.5 Cytochrome C 12,400 10.5 Serum albumin 66,000 5.3 B-Amylase 200,000 5.7 Apoferitin 443,000 4.0 Fill in the blank. If the mixture of proteins above was subjected to anion exchange chromatography at pH 8, from the column. would be the first protein to elutedraw acyl coa derived from docosanoic acid C21H43CO2HOptimum temp of amylase was identified by incubation of the amylase together with starch at various temps for 10 mins. and stopped with HCI. The volume of added DNS is negligle. Volume Volume Volume Concentration of Temperature (°C) of of stock of stock starch distilled Absorbance amylase starch (mg/mL) water (uL) (mL) (mL) 1 1 10 9 0.0053 10 1 1 10 9 0.1309 20 1 1 10 9 0.1185 30 1 1 10 9 0.0531 40 1 1 10 0.0206 Using this graphically determine the optimum temp of amylase?
- Handwritten Identify the molecule names, enzyme name, enzyme classification and change in reaction(for glycolysis pathway)Enzyme Triacylglycerol Lipase Rennin Catalase Hexokinase Enzyme Official Name (write N/A if not applicable) Enzyme Official Number (4 digits) Simple or Conjugated Enzyme (specify cofactor if applicable) Type of Reaction Catalyzed Substrate Optimum pH Optimum Temperature Function/s Disease (give 1 and describe briefly)You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below. Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase 45% Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Glutamic dehydrogenase? © 20% O 30% 45% 65% 80%
- Glutamate is derived from which of the following molecules? oxaloacetate pyruvate O 3-phosphoglycerate chorismate O a-ketoglutarateFor myristic acid, C 13H 27CO 2H: (a) How many molecules of acetyl CoA are formed from complete β-oxidation? (b) How many cycles of β-oxidation are needed for complete oxidation?What is the catalytic efficiency of Catalase ? Table. The values of KM and kcat for some Enzymes and Substrates Enzyme Carbonic anhydrase Substrate CO2 HCO3 KM (M) 1.2 x 10-2 2.6 x 10-2 Kcat (s-1) 1.0 x 106 4.0 x 105 Catalase H2O2 2.5 x 10-2 1.0 x 107 Urease Urea 2.5 x 10-2 4.0 x 105 O A. 4 x 108 M-s-1 O B. 4 x 108 M-1.s-1 OC25x 10-9 M-s1 D. 2.5 x 102 M-1.s-1 OE 1.0 x 107 s1
