[2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of 0.1M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCI
[2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of 0.1M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCI
Chapter13: Aquatic Structures And Equipment
Section: Chapter Questions
Problem 4KA
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Question
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9:28
amino_acid_titration.pdf
Method:
Pipette 10 ml of alanine solution (0.1 M) into a 50 ml breaker.
Add 0.5 ml of (0.1 M) HCI from the burette and determine the pH of the solution after
each addition.
.
Continue adding acid in until pH falls to about 1.3.
Wash the electrode in distilled water titrate a further 10 ml of alanine solution with 0.1
M NaOH until pH reaches 12.5.
Plot a titration curve for alanine (pH verses titrant in ml).
Do the titration of arginine in a similar way and plot their titration curves.
Result:
Record the titration table and Plot a Curve of pH versus ml of NaOH and HCI
added.
Q
Determine the pka and pl values from your curves and compare them with the
standard values.
For alanine: pK,= 2.34, pK₂= 9.69, pl= 6.01
For arginine: pK₁= 2.17, pK₂= 9.04, pK₂= 12.48, pl= 10.76
B
Discussion :
[1] Calculate the pH of the alanine solution after the addition of Oml, 5ml of
0.1M NaOH and calculate pH after addition of 1ml, 2.5ml of HCI
[2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of
0.1M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCI
Dashboard
[3] Compare your calculated pH values with those obtained from Curve.
[4] At what pH-range did the acid show buffering behavior? What are the
chemical species at that region, what are their proportions?
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Transcribed Image Text:<
9:28
amino_acid_titration.pdf
Method:
Pipette 10 ml of alanine solution (0.1 M) into a 50 ml breaker.
Add 0.5 ml of (0.1 M) HCI from the burette and determine the pH of the solution after
each addition.
.
Continue adding acid in until pH falls to about 1.3.
Wash the electrode in distilled water titrate a further 10 ml of alanine solution with 0.1
M NaOH until pH reaches 12.5.
Plot a titration curve for alanine (pH verses titrant in ml).
Do the titration of arginine in a similar way and plot their titration curves.
Result:
Record the titration table and Plot a Curve of pH versus ml of NaOH and HCI
added.
Q
Determine the pka and pl values from your curves and compare them with the
standard values.
For alanine: pK,= 2.34, pK₂= 9.69, pl= 6.01
For arginine: pK₁= 2.17, pK₂= 9.04, pK₂= 12.48, pl= 10.76
B
Discussion :
[1] Calculate the pH of the alanine solution after the addition of Oml, 5ml of
0.1M NaOH and calculate pH after addition of 1ml, 2.5ml of HCI
[2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of
0.1M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCI
Dashboard
[3] Compare your calculated pH values with those obtained from Curve.
[4] At what pH-range did the acid show buffering behavior? What are the
chemical species at that region, what are their proportions?
Calendar
2
To Do
Notifications
Inbox
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