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explain how the use of an Eosin methylene blue agar plate can help determine the type of fermenters that bacteria are. explain throughly.
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- Describe how specimen collection is performed using aseptic techniques to isolate medically significant bacteria and identify these clinically important bacterial species, Escherichia coli, Streptococcus pneumonia, Haemophilus influenzae and Streptococcus pyogenes by using different staining techniques and biochemical tests. Evaluate the effectiveness of different biochemical techniques used to identify different bacterial species.Describe the procedure for obtaining the minimuminhibitory concentration (MIC) for a chemical that isbactericidal for Escherichia coli. Contrast the action ofdisinfectants and antiseptics.Discuss the importance of some bacteria to undergo starvation during stationary phase and importance of knowing the right culture media for specific microorganism.
- Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agara.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixture
- Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarAre the large numbers of microorganisms found in the mouth cause for concern? Explain. How can you determine whether a culture or subculture is pure? What kinds of clinical speciments may yield a mixed flora in bacterial cultures? Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
- Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?MacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.Explain why it is essential that bacteria cultures be sterilized in an autoclave.