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- Calibri Light (H.. v 11 A BIU ov Av A 2. Are bacteria unit- or multicellular? What about the chains or colonies seen in slides and. ヘ三 diagrams? Explain. A. Bacteria also play a role in the maintenance of healthy ecosystem. They help decompose decaying matter and help digest food in the guts of animals. 3. If a person is diagnosed with syphilis, which bacteria species is most likely the cause? A. Syphilis is usually a sexually transmitted disease, but it is occasionally acquired by direct nonsexual contact with an infected person, and it can also be acquired by an unborn fetus through infection in the mother 4. Think about Clostridium botulinum. Explain the terms below that describe this species. a. Gram-positive b. Anaerobic C. Motile d. Botulism 5. Explain why methicillin-resistant S. aureus (MRSA) is a problem in clinical medicine. 6. Do you see any nuclei in the specimens? What accounts for the colors of the cells? Explain. 7. Explain gram stain method in terms of how useful it is…Search ew View Help O Editing v BIUQv Av A ニv三マ 川 ... permitting the growth of gram positive bacteria. Is phenylethyl alcohol agar a differential or selective media? Selective media 5. A strain of Moraxella catarrhalis is able to grow on agar containing the antibiotic amoxicillin. You suspect this strain may contain the enzyme B-lactamase which inactivates amoxicillin, making it ineffective as an antibiotic. You grow the same strain in media containing amoxicillin and clavulanic acid (a B-lactamase inhibitor). What do expect the results to be; growth or no growth? Explain. s: On ENG 103 Project 1...pdf PDF Getting starte Personal Narrative..pdf PDF PDFAnswer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
- use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30Antibiotic Code Zone of Inhibition (mm) S, I, or R? Penicillin P 15 Streptomycin S 8 Ampicillin A 25 Chloramphenicol C 14 Bacitracin B 5 QUESTIONS: Which antimicrobial agent was most effective against your organism? Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not? Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain. 4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test: Antibiotic Zone of Inhibition (mm) Astonostatin 12 Bodaciosporin 25 Crazifloxacin 5 Dorkimycin 16 When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result. Based purely on these…The following results were obtained from a disk diffusion test for a strain of Escherichia coli. Chemotherapeutic Zone of inhibition (mm) A 7 B 10 C 18 D 3 Which drug above (A, B, C, or D) is most effective against this E. coli strain? ***Which drug above (A, B, C, or D) is most effective against Staphylococcus aureus? Explain your answer.
- A Fig. 2 Result showing the effect of different germicidal soap on the growth of bacteria Guide questions: 1. Based from figure 2 which soap is most effective? Explain 2. Which is least effective? Explain 3. Explain the result of disc D.The production of a product P from a particular gram-negative bacteria follows the Monod growth law max Cs Co Ks + C, rg = with max (a) The reaction is to be carried out in a batch reactor with the initial cell concentration of Cco = 0.1 g/dm³ and substrate concentration of Cso = 20 g/dm³. = 1 h-¹, Ks = 0.25 g/dm³, and Y₁ = 0.5 g/g. c/s = p/c Cc Cco + Yc/s(Cs0 - Cs) = Plot rg, -rs-re, Cs, and Co as a function of time. The reaction is now to be car (b) dilution rate at which wash-out out in a CSTR with Cso = 20 g/dm³ and Cco = 0. What is the occurs? (c) For the conditions in part (b), what is the dilution rate that will give the maximum product rate 0.15 g/g? What are the concentrations Cc, C₁, Cp, and ―r, at this value of D? (g/h) if Y8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?
- 1). SIM Tubes and TSI slants turn black if the organism is able to reduce iron compounds. For which organism(s) below would you expect a black reaction? Circle all that apply. Escherichia coli. Proteus vulgaris. Salmonella typhimurium. Alcaligenes faecalis. Shigella flexneri. 2). If Citrate is a sugar and fermentation generates an acid, why does the BromThymol Blue indicator in the media turn blue and not yellow? 3). Why is Salmonellosis associated with eggs? Can you get Salmonellosis from other animal sources?Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?1. To test the ability of each diluent to sustain the viability of Escherichia coli during dilution, the cells were incubated at room temperature (20 to 23°C) or 37°C for 0 or 2 h and plated on NA agarin duplicate. The counts obtained are shown in the following table: E. coli counts on dilution time O hour 2 hours Diluents 10-5 10-6 10-7 10-5 10-6 10-7 R1 26 ; 27 3:2 0:0 0:0 0:0 0:0 Distilled Water R2 31 ; 27 1:0 0:0 0:0 0:0 0:0 (DW) R3 27 : 28 4:5 0:0 0:0 0:0 0:0 R1 290 : 295 34 :31 2:1 252 : 239 27 : 26 1;1 DW-0.85% NacI R2 315 ; 309 37 : 34 2;2 250 : 245 26 ; 26 1:0 R3 301 ; 285 36 : 44 1:2 235 ; 267 34 : 21 0:0 R1 385 ; 392 42 : 35 4:5 368 :379 33 ; 32 2:2 DW-3.0% Nacı R2 370 : 388 37 : 40 2:4 305 : 303 35 ; 30 2:5 R3 355 : 365 35 : 28 4:6 370 :350 33: 37 3:1 R1 380 ; 383 30 : 27 2:2 255 : 245 25 : 24 2;2 PBS R2 341 ; 352 27 : 34 1:3 230 : 245 26 : 27 2:2 R3 329 ; 335 33 : 31 3:6 242 : 250 28 : 29 2:1 R1 340 ; 320 32 : 35 1:0 245 : 249 27 : 25 1 1 PBS-0.85% Naci R2 340 ; 345 35 :…