Describe the reason why bacterial population in a culture tends to grow slowly under unfavorable conditions like the presence of poison or antibiotics. Also, explain how the population is restored in the same adverse conditions in the same culture, in due course of time.
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Population Growth
R and K Selection
R and K selection are concepts in ecology used to describe traits in the fluctuation of a population or population dynamics. For example, they describe the life-association traits between parent and offspring, such as quantity or number of young ones born at a time, quality of parental care, the age to maturity, and reproductive effort.
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- An attempt to transfer bacteria into new media during the death (Decline) phase of a culture resulted in actual growth of the organisms. What is the most likely explanation of this phenomenon?A microbiologist inoculates a growth medium with 100 bacterial cells/ml. If the generation time of the species is 1 hour, and the microbes are in the exponential (log) phase of growth, how many hours require for the culture to surpass 10,000 cells/ml, if the culture is checked once each hour? O 1) 2 hours O 2) 7 hours O 3) 3 hours O 4) 10 hours O 5) 24 hoursDescribe and give a specific example of a facilitateddiffusion process.
- After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.Based on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.An inoculum of 106 bacterial cells was introduced into a flask of culture medium and growth monitored. No change was seen for 18 minutes (the lag phase), then growth occurred rapidly. After a further 87 minutes, the population had increased to 5.08 × 107 cells. What is the doubling time of the culture? Show your solution. (The 87-minute period is after the lag phase.)
- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?In stages of bacterial growth, this is the phase where the bacterium adjusts to the new environment and the start of biosynthesis although increase in cell mass is not evident. Question options: Period of Rejuvenescence Log Phase Plateau Phase Exponential PhaseMALDI-TOF, is a method for identifying bacteria quickly. Look at this graphic then put the steps in order. Please follow a pathway of steps involving the Bacterial Culture: Get a readout of species identification and (sometimes) whether it is resistant or susceptible to antibiotics. Use colony from a nutrient agar plate. Prepare the sample, run it through MALDI-TOF machine. Grow a clinical sample on nutrient agar.
- explain how the use of an Eosin methylene blue agar plate can help determine the type of fermenters that bacteria are. explain throughly.What are the defining characteristics of the exponential/log phase of the bacterial growth curve. Explain the culture conditions that arise that cause the phase to end. In different culture media, and the exact same starting inoculum, will the end of exponential/log occur at the same time? Why or why not? A graph or a drawing of the growth curve would help but is not essential to answer this question.The UWC Biotechnolo class undertook a study to determine the microbiological quality of mince-meat samples that were obtained from four local retail outlets. You have performed the following serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferred to 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3) 100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the required medium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the table below: 1 3 4 6. Total Viable Count >300 >300 >300 274 28 3 >300 >300 >300 286 29 3 Mold and Yeast Count >300 >300 129 14 2 >300 >300 127 13 1 Staphylococcal Count >300 168 18 3 10 1 >300 156 16 2 Enterobacteriaceae >300 >300 264 27 Count >300 >300 248 25 2 a. Determine the log colony forming units per gram of product for the different counts. b. If the allowable microbiological standard for the Enterobacteriaceae count is…