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2. As an aspiring Nurse, the need for the culture of a new pathogenic microorganism in the laboratory has been allocated to you. Applying all the guidelines involved in the successful isolation and cultivation of microbes, discuss the culturing system that is most appropriate for this activity and state your reason(s) for this choice
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- Please help me to provide an introduction to the significance of pesticides and antibiotic resistance in aquaculture. This is for my presentation. Thank you very much1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.A nurse is following the principles of medical asepsis whenperforming patient care in a hospital setting. Which nursingaction performed by the nurse follows these recommendedguidelines?a. The nurse carries the patients’ soiled bed linens close tothe body to prevent spreading microorganisms into the air.b. The nurse places soiled bed linens and hospital gowns onthe floor when making the bed.c. The nurse moves the patient table away from the nurse’sbody when wiping it off after a meal.d. The nurse cleans the most soiled items in the patient’sbathroom first and follows with the cleaner items.
- 2. Make a viable count of the donor culture by spot inoculation of the nutrient agar plate WITHOUT antibiotics as follows: • Prepare a 10 fold serial dilution (to 10-7) of the DONOR culture in sterile saline. • Show clearly how you intend to prepare this dilution series and have it checked by a demonstrator before continuing. Dilution Volume of saline (units?) Volume of 10-1 10-2 10-3 104 10-5 10-6 10-7 which other | dilution? • Clearly label the base of the nutrient broth agar plate as shown in the diagram below. Include your initials, the date, and what the plate contains (donor viable cell count). • Carefully dispense 3 x 10 µl drops of the 104, 10-5, 106 and 10-7 dilutions of the culture within quarter areas of the agar plate then replace the lid. Allow the inocula to soak into the agar before inverting the plate for incubation. • What is the purpose of this step? 4935. There is a mystery and an unusual bacterial disease in Contra Costa County. Researchers as well as physicians conclude that it must be controlled before it goes out of hand. They also find that the pathogen has a extended generation time. What antibiotic do you suggest to use? Group of answer choices Rifamycin Penicillin Quinolones ( such as ciprofloxacillin) Tetracycline Sulfa drug and trimethoprim combination 36. Which of the following best describes action of the ciprofloxacillin in prokaryotes ? Group of answer choices inhibits RNA polymerase, consequently no RNA synthesis in bacteria interferes with DNA replication by randomly nicking the DNA enhances DNA gyrase activity in killing UTI pathogens binds and modifies elongation factor ( ADP ribosylates) inhibits prokaryotic translation by binding to 50S subunits and arresting translocation inhibits DNA gyrase; prevents snapping back of the separated strands at replication forkYou are required to completed an in-depth study of a common disease bacterial spot affiliated with the production of legumes.- o Causal Agent o Symptoms/ Damage o Range of Crop Affected o Control Measures
- The Centers for Medicare and Medicaid Services (CMS) have initiated revised policies regarding reimbursement to hospitals for care of patients who suffer health care-associated infections (HAIs) such as SSIs. Hospitals must bear the costs of treatment. Health care workers are the key in preventing patient injury and protecting hospitals. What is an example of a routine procedure performed by surgical technologists prior to entering the sterile field that would be part of the aseptic technique?Scenario 1. Microbial examination of Food (Aerobic Plate Count) Fifty grams of food sample (vegetable dish) is collected from a cafeteria in Area 2, UP campus using sterile spatula. The sample is transferred into sterile containers and transported to the laboratory immediately after collection. In the laboratory, 450 ml of diluent (phosphate-buffered water) is added to the sample. The sample is blended in the stomacher to ensure homogenization. Ten mL aliquot of the sample is transferred to 90 mL phosphate buffer, followed by another two 10-fold dilutions. The different dilutions are spread plated in triplicate PCA plates and incubated at 37 °C for 24-48 hours. Only the last three dilutions were plated. Colony counts were recorded as shown in Table below: Dilution/ Dilution 2 Dilution 3 Dilution 4 Trial Plate count (cfu) Trial 1 315 104 17 Trial 2 225 81 15 Trial 3 210 93 20 1. Illustrate the procedure done by the student. 2. The total Aerobic Plate Count is intended to indicate the…Which of the following definitions is true about Contamination TERM? a. The mixing of some material with the plant by accident b. The study of plant origin, morphology of active ingredient, pharmacological effects and how to extract and store c. Drug that match the characteristic in the pharmacopoeia d. The study of the traditional use of plants for medicinal purposes
- 1. What is the key ingredient of hand sanitizers?  include a image of the chemical structure of the ingredient and explain how it works 2. What did Ignacio Semmelweis discover? Describe the situation and what he recommended to the medical community of 1 stion s page 30- F2 Which of the following terms is associated with the probability of a preparation not being sterile after it has been exposed to a sterilization process? Select one: Oa. Particulate Matter level Qb. Sterility Assurance level О с. Pyrogen Load level O d. Optimal Sterilization level F3 F4 F5 Ol پیار Q Search F6 F7 F8 F9 === 8 9 F10 Time left 22:34:03 F11 Next pageYou have provided patient teaching for a patient you are discharging home on an antibiotic. What statement by the patient indicates to the nurse that additional teaching concerning the use of antibiotics is needed? O "Antibiotics will not help me when I have a viral infection." O "A bacterial culture will be done before antibiotics are prescribed for me." O "I could develop diarrhea as a result of taking an antibiotic." O "I will stop the antibiotic as soon as I feel better."
