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Differentiate between surface immobilisation and microencapsulation of enzymes and discuss the advantages and disadvantages of each method.
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- A) Draw and label the set-up of Manual paraffin wax infiltration. B) Answer the following questions:1. Differentiate the 3 types of tissue Impregnation in terms of: 2. Impregnation Techniques Advantages Disadvantages A. Paraffin Infiltration B.Celloidin Infiltration C.Gelatin Impregnation C) What is the required volume of embedding medium for routine tissue processing? D) What is Plastic embedding? What is Double embedding?Define the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?
- Outline in detail the procedure for celloidin embedding .After a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE:Differentiate between microscopic and macroscopic methods ofobserving microorganisms, citing a specific example of each method.
- What is the main group of microorganisms producing the most antibiotics? Describe the biochemical characteristics of this microorganism and provide TWO (2) examples of antibiotics produced by these microbes.Briefly explain that what is the important to use immobilization techniques in biosensor?..(explain under5 points)Patch clamp What is patch clamp technique? Identify two features of the patch clamp technique that make it a useful tool in studying cellular electrophysiology. Explain each thoroughly. What is the role of the reference electrode in the patch clamp technique? Discuss how it works.
- Explain in detail the methods that can use to achieve static and or cidal effects in microorganismsIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperAseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.