a) In the flow cytometry experiment, explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should be included in the experimental design. (30%)

a) In the flow cytometry experiment, explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should be included in the experimental design. (30%)

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter42: Animal Development

Section: Chapter Questions

Problem 3CT

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Concept explainers

Sleep Physiology

Sleep is a condition of reduced muscle movement in the body. Awareness about the surroundings is reduced at the time of sleep. The body stays mildly responsive or non-responsive to external stimuli. The sleep-wake cycle is controlled by the circadian rhythm that produces a resting phase for the body.

Question

2. For a fictional human tissue, it is hypothesised that cancer stem
cells have the following expression pattern of cell surface markers
(CD44+/CD24+/ESA+). Flow cytometry was used to isolate this
population of cells.
COMM
CD24
ESA
1%
Tumour volume (cm³)
a) In the flow cytometry experiment, explain how to determine
what percentage of the total cell population are the putative
cancer stem cells. Also, briefly describe two controls that
should be included in the experimental design. (30%)
3.01
2.5-
+602
In a follow-up experiment, sorted and unsorted cells were
injected subcutaneously into immunodeficient mice. The
following plot depicts the results from the experiment.
2.0-
1.5-
1.0-
CD24
0.5-
ESA
37%
• Unsorted
CD44+/CD24+/ESA+
CD44+/CD24-/ESA-
Time (weeks)
10 12

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