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- Describe an experiment that lead to the establishment of the fact that DNA replication is semiconservativeIllustrate the mechanism of a semiconservative model of DNA replication.a) Explain semi-conservative modelmof DNA replication b) discuss initiation in DNA replication and explain why it is a tightly controlled process.
- In which thing the experimental proof for semiconservative replication of DNA was first shown?What are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication. Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.Describe the early models of DNA replication that were investigated and explain how research by Meselson and Stahl supported the current model . Explain the role of each of the following in DNA replication. - helicase - Gyrase - RNA primer - DNA polymerase - Okazaki fragments - DNA ligase
- With illustrative diagrams, explain the three theories of DNA replication..Cite evidence from Meselson and Stahl’s experiment that enabled scientists to differentiate between semiconservative replication of DNA and alternative models.How did the results prove the semiconservative model of DNA replication? Explain
- Name an important difference in the replication of circular DNA versus linear double-stranded DNA.You are studying a colony of cells and determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. A) What will the effect of the mutation in DNA polymerase I be on DNA replication? In your answer make sure to describe what would be observed in the leading and lagging strand and explain your reasoning. B) Will this mutation in DNA polymerase I have an impact on another step in DNA replication? In your answer make sure to indicate whether DNA replication will be impacted or not. If it is not, explain why. If it is impacted, then describe the step that is impacted and name the molecule or enzyme involved.Describe three (3) effects on DNA replication if DNA polymerase could build DNA in both directions – in both the 5’ to 3’ direction and the 3’ to 5’ direction.