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Define inoculation, media, and culture, and describe sampling
methods and instruments, and what aspects must be controlled.
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- Define the term sterile and explain why sterile instruments and media are necessary to be used in the laboratory.Provide a brief description and state the function of the following tools and equipment used in the microbiology laboratory. Incubator Hot air oven Centrifuge Spectrophotometer Magnetic stirrer Orbital shakerExplain why specimens should be taken aseptically and processed quickly to ensure accurate results, even when nonsterile sites are being sampled and selective media are to be used.
- Outline the most signifi cant discoveries and events in microscopy, culture techniques, and other methods of handling or controlling microbes.Explain several techniques in specimen collection and theconcerns in acquiring good cultures.Explain why is it important to follow proper precautions and use aseptic technique when working with clinical samples.
- Describe the use of microscopy to identify microorganisms in patient specimensProvide a brief description and state the function of the following tools and equipment used in the microbiology laboratory. o Commmon glassware ( test tubes, culture tubes and screw capped tubes ) o Petri dish o Pipette o Glass spreader o Inoculation needle o Inoculation/transfer loop o Bunsen burner o Pressure cooker o Incubator o Hot air oven o Centrifuge o Spectrophotometer o Magnetic stirrer o Orbital shaker o Hot plate o Water bath o Carbon dioxide cylinder o Single pan balance with weights o Chemical balance o Ph meter o Colony counter o Electrophoretic apparatus o autoclaveExplain some of the problems involved in sterilizing delicate andheat-sensitive instruments such as endoscopes, syringes, needles,catheters, and other instruments.
- Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *Identify one hazard and one associated risk present in an actual microbiology laboratory.What situations would result in recollection or rejection of specimen? Determine corresponding solutions to avoid such conditions.