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- A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown below. What other dye/s are present in dye C? Which dye contains the least number of pigments?A student performed a chromatography experiment to determine the Rf value of an unknown amino acid. The distances traveled by the amino acid and the solvent line were 3.0 cm and 8.5 cm, respectively. What is the Rf value for this amino acid? 0.3 cm O 0.35 1.8 5.5 0.85 cm 0.65 O 2.83. Correlate the structures of the standard food dyes to their relative position in the paper chromatogram. Refer to the structures shown below. *Na O H3C- OISI *Na HO CH3 Allura Red AC (pigment in red dye) N Hora O Na* O Na* O Nat O Na+ 0=8=0 HO 0=S=0 -O Na+ O Na+ Tartrazine (pigment in yellow dye) Brilliant Blue FCF (pigment in blue dye) 4. What are the identities of the unknowns in paper chromatography of food dyes? How did you arrive at this conclusion?
- PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.A student analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. OH Hollo HO OH OH OH NH₂ NH₂ NH₂ Threonine Valine Glutamic acid a. Identify spots A, B, and C in the developed TLC plate below based on the structures of the amino acids above (ie. which spot is threonine, valine, glutamic acid?). Hint: determine how the structures of the given amino acids differ A. BO С. b. Threonine can be phosphorylated using an enzyme called protein kinase. Given the same conditions the student used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Which of the following compounds would have the lowest Rf value in a TLC analysis? A. Hexane-2,3-diol B. Benzene C. Diethyl ether D. 1,3,5-cyclohexanetrioluse the following chromatogram and table as your data. What is the %composition of the Vitamin A peak in the sample? 78.43% 77.73% 7.867% 72.74%
- Atomic weights are not provided, but please use rounded-off values to the nearest whole number.(examples: 24.305, use "24"; 107.8682, use "108"; 1.00784, use "1"; 15.999, use "16") Final answer in 3 decimal places 1. A fat sample with combination free fatty acids required 8mL and 5mL of standard hydrochloric acid for blank and sample titration, respectively. The normality of the standard hydrochloric acid is 0.93N and the weight of the sample is 3 grams. Calculate the saponification value. 2. A 500mg oil sample is taken from a conical flask and is dissolved in 50mL distilled alcohol. An indicator is added and is then titrated against 0.112N KOH until a slight pink color appears. It took 17.6mL of the titrant to reach the endpoint. What is the sample’s Acid Value?Consider the following molecules. Which statement(s) is TRUE? CH3 CH3 но ČH3 но ČD3 i. You could differentiate between the two with a spot test. ii. You could differentiate between the two with liquid chromatography. iii. You could differentiate between the two with TLC. iv. You could differentiate between the two with a microcrystalline test. O a. i and iv only O b. ii and iii only O c. iii only O d. i only e. Neither i, ii, iii, nor iv are correct statements.Identify the detectors in gas chromatography that can be used for the detection of each type of analyte. More than one detector may be appropriate for a given analyte, and a given detector may be appropriate for more than one type of analyte. compounds ionized by UV radiation sulfur or nitrogen containing compounds halogenated compounds flame ionization Answer Bank mass spectrometer nitrogen and phosphorous containing compounds thermal conductivity electrolytic conductivity hydrocarbons electron capture thermionic photoionization
- 1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?In spectrophotometry, does the sample tested contain the allowable concentration of Allura red as prescribe by the FDA?An oil sample had its iodine number determined using the pyridinium tribromide method. The sample and reagents used are as follows: Oil sample: 0.19 mL, density: 0.954 g/mL Pyridinium tribromide: 0.472 g Na2S203: 0.1 M, endpoint 27.68 mL %3D What is the approximate Bromine Number? O 138 89 O 120 O 200