Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA. What is one explanation for why RNA could be present in the sample that should have contained plasmid DNA only? Think about the chemical similarities of these two molecules.

Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter5: Abbreviations And Systems Of Measurement
Section: Chapter Questions
Problem 5CRQ
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Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA.

What is one explanation for why RNA could be present in the sample that should have contained plasmid DNA only? Think about the chemical similarities of these two molecules.

plasmid DNA
RNA
PUC18
pBR322
RNase TE DNase RNase DNase TE
1
2
3
5
6
location of blue dye front
Transcribed Image Text:plasmid DNA RNA PUC18 pBR322 RNase TE DNase RNase DNase TE 1 2 3 5 6 location of blue dye front
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