A scientist transfers 0.1 mL phage stock to a tube containing 9.9 mL tryptic soy broth. She then transfers 0.1 mL from that tube to a second tube containing 9.9 mL. She then plated 0.1 mL of the content in the second tube. The total fold of dilution is:

A scientist transfers 0.1 mL phage stock to a tube containing 9.9 mL tryptic soy broth. She then transfers 0.1 mL from that tube to a second tube containing 9.9 mL. She then plated 0.1 mL of the content in the second tube. The total fold of dilution is:

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 2RQ

See similar textbooks

Related questions

Q: Please help with this and explain in detail. Please list the differences and similarities between…

A: The main differences and similarities between the lectin, alternative, and classical complement…

Q: Why do you think there was so much emphasis put on those initially? How does it relate to the organ…

A: 1. Emphasis on Cells and Organelles: - Cells are the fundamental units of life, and organelles are…

Q: You are studying a population of 500 sage grouse. You assay the population for diversity at a locus…

A: The new allele found in just a single bird shows a low frequency in the population. To work out the…

Q: How might the inclusion and engagement of biotechnology and pharmaceutical companies in the cancer…

A: The inclusion and engagement of biotechnology and pharmaceutical companies in the cancer planning…

Q: I need help counting how many stomata there is in this sample.

A: Small controlled pore complexes called stomata are found in the outermost layer of leaves and other…

Q: Draw the asexual and sexual phases of diatom reproduction. Be sure to include the decreasing sizes…

A: Diatoms are a type of Chrysophyte algae which is eukaryotic and unicellular. The main mode of…

Q: In humans, the ability to roll the tongue is a dominant trait (R), while the inability to roll the…

A: A square diagram called a Punnett square is used to forecast the genotypes of a certain cross or…

Q: Some Aleutian Islands are surrounded by urchin barrens while others are surrounded by kelp forests.…

A: The objective of the question is to identify the primary reason for the difference in the ecosystems…

Q: When I look back on all these worries, I remember the story of the old man who said on his deathbed…

A: Reflective prompts like this one encourage reflection and thought on the nature of stress and…

Q: Fruit flies (Drosophila melanogaster) are a common model organism in genetics labs. A somatic cell…

A: Fruit flies, or Drosophila melanogaster, are frequently used as model organisms in genetic research…

Q: Helping a student w this can you help with the diagram? Her answer is fine I need help with the…

A: The objective of the question is to understand the role of primers in a PCR reaction, how they can…

Q: Which marine zone would have the lowest total rate of photosynthesis? photic littoral…

A: The objective of the question is to identify the marine zone with the lowest total rate of…

Q: Individuals with the recessive trait of night blindness (genotype nn) have difficulty seeing in low…

A: The objective of the question is to determine the possible genotypes of the parental generation…

Q: Describe the impact of team development on a cancer program plan

A: The objective of the question is to understand the influence of team development on the planning and…

Q: How is a bone marrow differential performed?

A: The objective of this question is to understand the process of performing a bone marrow…

Q: Refer to the diagram below, which is representative of a single eukaryotic gene where the top strand…

A: Although DNA is double-stranded, transcription uses only one strand as a template at a time. The non…

Q: Draw a mechanism to account for the inter-strand cross linkage of two guanines by busulfan. Explain…

A: DNA is the fundamental hereditary molecule in almost all living organisms, carrying the genetic…

Q: Which of the following is a measure of CPE from a viral infection? 1. End point dilution assay 2.…

A: The objective of the question is to identify the correct methods used to measure Cytopathic Effect…

Q: To determine an association between smoking cigarettes and the development of prostate cancer among…

A: We can use the following formula, to calculate the odds ratio for the association between smoking…

Q: B cells - A comparison of B and T cell maturation. List the locations and what is happening…

A: B and T cells, fundamental components of the adaptive immune system, undergo a structured maturation…

Q: Question 2: Now complete the sketch for an XY human male cell undergoing spermatogenesis if a…

A: Keeping track of the sex chromosomes, this explanation will walk you through the process of…

Q: 2. SRY: What is the role of this gene in sex determination? How does this gene explain the function…

A: Primary sex determination in animals is mostly determined by chromosomes and is often unaffected by…

Q: Table 1. Gametophytes of mosses, ferns, and flowers. Nonvascular or vascular Vascular Seedless or…

A: A gametophyte is a haploid multicellular stage in the life cycle of plants and some algae. In…

Q: protein must be conformationally stable protein may be denatured analysis requires information about…

A: Protein structure, function, and characteristics are only fully understood through the use of…

Q: A young couple went to see a genetic counselor because each had a sibling affected with cystic…

A: Cystic fibrosis is an example of autosomal recessive disorder. The recessive mutant allele is…

Q: How is the construction of large icosahedral viral capsids different from small icosahedral viral…

A: The objective of the question is to understand the differences in the construction of large…

Q: Use the information provided in the experiment below to annotate/ label the figure. Background: Boll…

A: Boll weevil poses a significant threat to cotton crops, leading to considerable economic losses and…

Q: Ethanol is one example of alternative fuels for powering our cars and trucks. Ethanol can be…

A: The question is asking for the advantages and disadvantages of producing and burning ethanol as an…

Q: How can adhesion, cohesion, and surface tension work in plants?

A: The question is asking about the role of adhesion, cohesion, and surface tension in plants. These…

Q: How are sex-linked recessive phenotypes passed between generations? options: A) From father to…

A: Sex-linked recessive phenotypes are the traits which are determined by genes located on the sex…

Q: Tamoxifen is the cancer drug you read about at the beginning of the lesson. Predict five bonds that…

A: Cancer drugs (Tamoxifen) is used in cancer treatment to stop uncontrolled cell growth.Polar bonds…

Q: In a cross between a black and a white guinea pig, all members of the F₁ generation are black. The…

A: For the question we need to make punnet square, which is a diagram which shows the possibilities in…

Q: In beetles, an X-linked gene determines body size, with normal size (M)completely dominant to…

A: A genetic cross is an experimental breeding process performed to understand the patterns of…

Q: An (Ab)/(aB) heterozygote produces gametes. The recombinant gametes will have these recombinant…

A: Recombination is a process that occurs during meiosis where the genetic material exchanges places…

Q: Here, N₁ is the number of fish with genotype II, Nic the number with genotype Ic, and Ncc the number…

A: To calculate the allele frequncies, we need to determine the total number of alleles and then divide…

Q: You are studying the inheritance pattern of hair color in troll dolls, which have XY sex…

A: Answer:- True-breeding blue-haired male (XY) crossed with true-breeding orange-haired female…

Q: Explain the specific evidence-based information and organizational data you needed to collect during…

A: Collecting evidence-based information and organisational data is crucial for making informed choices…

Q: Individuals increasing sweat production implies that they have adapted the change in temperature…

A: The question is asking whether an increase in sweat production in individuals implies that they have…

Q: O ■ Example: Suppose A₁ mutates to A2 at rate 0.005, but A2A2 homozygous recessives are 50% less fit…

A: Hardy Weinberg Equilibrium explains how allele and gene frequency remain same in the population from…

Q: Piebaldism is a skin condition that causes an infant to be born with white birthmarks due to a…

A: It is an autosomal dominant genetic disorder. It is mainly due to the absence of melanocytes of the…

Q: Use the information provided in the experiment below to annotate/ label the figure. Background: Boll…

A: Transgenic plants: Transgenic plants are those plants, which carry additional, stably integrated and…

Q: A business researcher is hired by a specialty retail firm. The retailer is trying to decide what…

A: The objective of the question is to identify the independent and dependent variables in a business…

Q: Most organisms' niches are controlled by Genetic trait for adaptive characteristics Luck Behavior…

A: Solution)The primary factors controlling most organisms' niches are-1) Genetic traits for adaptive…

Q: Infection rates for many STIs continue to be highest among young people (typically defined as around…

A: Understanding the variables contributing to high disease rates of sexually transmitted infections…

Q: LIZARD EVOLUTION VIRTUAL LAB Answer the following questions as you finish each module of the virtual…

A: “Since you have posted a question with multiple sub parts, we will provide the solution only to the…

Q: Dilution Factor 10,000 100,000 1,000,000 10,000,000 Plate colony count INTC* 613 120 18

A: Given:Data from standard plate counts (SPCs) including colony counts and dilution factors.It is…

Q: Several alternative definitions of the term species have been proposed, recognizing different…

A: The objective of the question is to determine whether the existence of multiple definitions of the…

Q: Identify the tissue from the endocrine system

A: The image shows a part of adrenal medulla gland. The adrenal medulla gland is present as an inner…

Q: two substances from the table above that would need to move into or out of a cell to maintain…

A: 1. Sodium (Na+):Although sodium ions (Na+) are important for nerve impulse transmission and muscle…

Q: Below is the DNA sequence (coding strand) for the human placental protein 14 (not all of the gene…

A: PCR stands for Polymerase Chain Reaction. It's a widely used laboratory technique in molecular…

Concept explainers

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.

Question

a) 100,000x

b) 1000x

c) 100x

d) 10,000x

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1: Total fold of dilution

VIEW

Step 2: Calculation

VIEW

Solution

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?
There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectively
A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?
You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?
You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
A culture of E. coli is diluted as follows:(1) 65mL are added to 435mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-3 dilution using 1000 uL from the original culture?
To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a serial dilution using three tubes of sterile saline (9.9 ml each). A sample of 0.1 ml from the culture was added to tube 1. Similarly, 0.1 ml from tube 1 was used to inoculate tube 2, and tube 3 was inoculated using 0.1 ml from tube 2. A nutrient agar plate was then inoculated using 0.1 ml from tube 3 and incubated overnight. The next day, 92 colonies were observed on the plate. How many cfu/ml were in the original culture? Using a Petroff-Hauser counting chamber, the number of cells in the same culture was estimated to be 8.5 • 109 cells/ml. How can you explain these results?
Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage? Show your calculations and give your answer in pfu/ml (pfu = plaque-forming units)
A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?
The volume of E. coli added to each warm agar pour/virus mixture was originally100µL. After pre-testing the experiment, the instructions were modified for you to add 300µL of E. coli. Make an educated guess as to why the volume was increased.
The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?