(A) Do electron transport chains create a gradient so that ATP synthase can generate ATP molecules? (B) What are the sources of energy that drive mitochondrial and chloroplast electron transport systems?
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- The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)18. The figures below illustrate the similarities between ATP synthesis in mitochondria and chloroplasts. Mitochondrion A NADH H,0 H* ΑΙΡ ADP Inner Mitochondrial Membrane ΔΔ Chloroplast H2O H+ NADP+ NADPH H*. AA ΑΙΡ ADP P; Thylakoid Membrane A Hydrogen ion O Electron The figures can best assist in answering which of the following questions? (A) Do electron transport chains create a gradient so that ATP synthase can generate ATP molecules? (B) What are the sources of energy that drive mitochondrial and chloroplast electron transport systems? (C) What is the optimal temperature at which ATP synthase chemically converts ADP and a phosphate group into one molecule of ATP? (D) What is the evolutionary relationship between the ATP synthase in mitochondria and the ATP synthase in chloroplasts?12. Use Figure 4 for questions a-c below. 114 ATP synthase matrix intermembrane space Figure 4: Sketch of a mitochondrion https://upload.wikimedia.org/wikipedia/commons/1/1a/Schema mitochondrion basic.svg Bionet, CC BY-SA 4.0 , via Wikimedia Commons a. Add to the sketch to show a H* concentration gradient in an active mitochondrion. (Sketch more H* in the appropriate space. Sketch less H* in the appropriate space.) 4+ b. Sketch an arrow to represent the direction that protons flow (via diffusion) through ATP synthase. c. If the concentration of protons was equal on each side of the membrane, how would that affect the flow of protons through ATP synthase? How would that affect ATP production from glucose?
- Referring to the figure below, explain why NADH yields more ATP than FADH2 does. Electron-transport and proton pump Oxidative phosphorylation Outer mitochondrial membrane H* -Intermembrane H+ H+ H+ space H* H+ H+ Cytochrome c H+ COQH, CoQ UU COQH2 CoQ JU U Inner mitochondrial membrane Ht e ATPase Complex II Complex II Complex IV Complex e ADP +P - Mitochondrial matrix NADH NAD+ FADH2 FAD АТР H+ -H+ H+ H20The following diagrams illustrate the two aqueous spaces and the inner membrane of a mitochondrion. In which diagram is the relative concentration of protons auch that ATP synthesis could occur if ATP synthase were present? (Gold spheres represent protons.) 圈圈圈圈圈 A B D E OA OB OC OD OE intermembrane space Anner membrane matrieIntermembrane Space Protein Complex of Electron H Carriers ATP Synthase Cyt C NADH NAD FADH FAD 2H + ,0, H,0 Mitochondrial Matrix ADP+ ATP Electron Transport Chain Chemiosmosis Oxidative Phosphorylation 19- Cyanide inhibits cytochrome c oxidase, a component of the electron transport chain. If cyanide poisoning occurs, would you expect the pH of the intermembrane space to increase or decrease? What effect would cyanide have on ATP synthesis? 20. Because they lose their mitochondria during development, red blood cells cannot perform aerobic respiration; however, they do perform glycolysis in the cytoplasm. Why do all cells need an energy source, and what would happen if glycolysis were blocked in a red blood cell? 21. What is the primary difference between a circular pathway and a linear pathway? 22. How do the roles of ubiquinone and cytochrome c differ from the roles of the other components of the electron transport chain? 23. What accounts for the different number of ATP molecules…
- How much energy is required to pump a mole of protons in a mitochondria with the following conditions? pH of matrix = 8.2, pH of intermembrane space 7.4, membrane potential of - 65 mV (N-side relative to P-side), temperature = 37C. Express answer to the nearest 0.1 kJ/mole.1. As has been discussed in class the electron transport chain is the first part of oxidative phosphorylation. This chain of multi-subunit enzymes is responsible for facilitating the transfer of electrons from NADH and succinate/FADH₂ to oxygen making water. Given this understanding answer the following questions. a. Below is an equation for determining the amount of energy required to move 1 mol of protons across the inner membrane of the mitochondria. In actively respiring mitochondria the A = +/-0.15 volts to +/- 0.20 volts. The ApH for a typically mitochondria will be 0.75 pH units, with the matrix (N-side) being more alkaline than the IMS (P-side). Given this state the total energy needed to move a single proton from the N to the P-side. Assume T = 310K and R = 8.314 J mol-¹ K-¹ and a A4 = 0.17 volts. Finally, assume F = 96.5 kJ/mol/volt AG = 2.3RTAPH + FA b. How much free energy is available in the transfer of electrons from NADH to oxygen given the following half-reactions and…How does DCPIP act as an indicator of presence of mitochondria? Will mitochondria in the presence of DCPIP still make ATP? Why or why not?
- 10 9 P production (mmoles/min) 8 7 6 5 4 Active 10 9 15. Based on what happens to the proton gradient, what happens to the production of ATP in oxidative phosphorylation in mitochondria affected by cyanide? 0 Intermembrane space Matrix 16. The graph below represents ATP production by an active mitochondrion (0-2 hours) before it is exposed to cyanide. Complete the graph below to show what you trend you expect to see with ATP production (2 - 5 hours) after cyanide is added. Effect of DNP on mitochondrial ATP production Status of the mitochondria Cyanide DNP Time (h)(c) Compare the differences between oxidative phosphorylation and photophosphorylation by redrawing (if necessary) and completing the table given below: Table 1: Comparison of oxidative phosphorylation and photophosphorylation Oxidative phosphorylation Photophosphorylation Organelle Source of electrons Final electron acceptor Source of energy Role of ATPThe figure below shows an illustration of a mitochondrian. Mustration of a mitochondrion Which of the following best describes the relationship between the structure and function of the mitochondrion? A B с D The shape of the mitochondrion Increases its material-exchange efficiency by maximiz ing its overall volume relative to its surface area. The double-membrane structure of the mitochondrion allows it to maintain its relatively acidic environment in which nutrients can be digested. The folds of the mitochondrion Inner membrane increase its material-exchange effi- ciency by maximizing its surface area relative to its volume. The specific structure of the mitochondrion Inner membrane allows it to correctly guide the folding of proteins into their functional shape.
