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A:
Q: Comparing chlorophyll a and b , which of the two traveled furthest up the tlc plate ? Why is that ?
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A:
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A:
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- In a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 b. Construct an appropriately labeled graph to illustrate the sample means of the two locations to within 95% confidence (i.e. sample mean + 2 SEM).A scientist is studying the enzyme X which is an important point of regulation in the metabolism of the inhabitants of Sumeru. He developed four Akademiyan-derived compounds which may possibly work against this enzyme, and tested using an eudiometer the metabolic rate of the sample cell lines. Data are below. Time for each compound (min) Eudiometer volume reading (mL) A B C D 0 83.1 62.6 89.0 66.4 5 83.2 100.6 90.2 71.4 10 83.2 83.6 95.0 77.2 15 83.2 83.6 100 84.6 20 83.3 110.8 104.6 88.2 Show the properly labeled volume versus time plot for each eudiometer, with the equation of the line and R2. What is the most effective inhibitor among the four compounds? It was found that the most effective inhibitor exhibits uncompetitive inhibition. Illustrate the Lineweaver-Burk plots of uninhibited and inhibited enzyme X with properly labeled axes.Botulinum Toxin identify the species which releases the toxin (if it is man-made then this will be all that is required for this part) identify the step disrupted in the neuromuscular junction pathway Provide any consequences of this disruption. Does the toxin have any applications in biomedicine as a painkiller, disease treatment or analgesic? Provide your source in APA format for each. If this is missing no credit will be awarded.
- Choose all that are correct regarding impeller type choice in bioreactors. O Marine type impellers are ideal for shear sensitive mammalian cells. O Marine type impellers are ideal for less shear sensitive yeast cells. O One function of the impeller is to mix the contents of the bioactor (e.g., nutirents). O None of these options are true. O Impeller type choice depends on the shear sensitivity of the target cells and the solution viscosity. Choose all that are true regarding the phases of cell growth in a bioreactor. O None of these options are true. O There will always be a death phase in the bioreactor, since some cells are dying throughout. O Higher variability in the bioreactor contents is associated with stationary phase. O Higher varaibility in the bioreactor contents is associated with exponential phase when the cells are growing most rapidly. O There will always be a lag phase of growth in a bioreactor since the cells need time to adjust to their new enviroment upon…Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.You plot a reaction progress curve and find the equation of the line to be y = -0.0961x + 0.7142. Calculate the U, U/μL and U/mL of lysozyme activity in this fraction. The lysozyme activity is done identical to what is indicated in the lab manual.
- In cultivation of E.coli strain in a batch stirred and aerated tank, a static gassing out technique method was employed to determine the oxygen transfer coefficient (k̟a) for the bioreactor. After purging the oxygen with nitrogen gas, continuous air supply at air flow rate of 0.1 v.v.m. and impeller speed of about 250 rpm was initiated. Increase in the dissolved oxygen concentration upon the continuous air sparging is recorded by a dissolved oxygen (DO) probe. Using the following data, determine the oxygen transfer coefficient (ka) for the bioreactor. Saturation DO concentration is C*=9 mg/l Time (minute) Cl (mg/l) 1 1 2 3 2.5 4 3 5 4 6.5 5 7.2Hexokinase in red blood cells has a Km of approximately 50 µM. What concentration of blood glucose would yield a velocity equal to 90 % Vmax? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 500 mM 0.0450 mM 4500 μΜ 5000 µM e 5.0 mM 0.50 mM 450 μΜ 4.5 mM 50 μΜ.The Quikchange cycling parameters are listed below: 1.95 degrees, 2 min 2. 95 degrees, 20 sec 3. 60 degrees, 10 sec 4. 68 degrees, 2 min 5. Repeat steps 2-4 18 times 6. 4 degrees, indefinitely Match each step above to the correct function(s) listed below. Write your selection(s) for each step. You may have more than one answer for each. 1.initial denaturation 2. dNTP pairing 3. renaturation 4. Primer annealing 5.new DNA extension 6. polymerase unwinding 7. refrigeration 8.secondary denaturation 9. degradation of excess DNA
- National Board of Medical Examiners Biochemistry Mark 36. In the presence of a metabolite (X), 6-phosphofructokinase is assayed at a fixed concentration of ATP and varying concentrations of fructose 6-phosphate. The resulting data are shown in the table. Fructose 6-phosphate (pM) 5 10 20 40 75 100 200 Velocity umoles/min 0.05 0.15 0.25 0.70 1.7 2.2 3.1 3.1 Velocity (+X) umoles/min 0.006 0.025 0. 10 0.35 1.03 16 2.9 3.1 400 Metabolite (X) is most likely which of the following substances? O A) ADP O B) AMP OC) CAMP D) Citric acid O E) Fructose-2,6-bisphosphateIn an enzymatic reaction, the equations that correspond with and without inhibition according to linewear-burk are calculated, these are with inh: 3.5x + 6 = y without inh: 3.5 + 10 = y. calculate alpha factorConsider the following experimental data from another experiment: [S] 1.5 2.00 2.50 5.00 10.00 V (No inhibitor) mmol ml¹ min¹ 0.167 0.204 0.232 0.313 0.385 V (inhibitor) mmol ml¹¹ min¹¹ 0.115 0.143 0.167 0.250 0.333 Calculate Km and V max and determine whether this inhibitor is competitive, non-competitive or uncompetitive.