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7 Why does incorporation of AZT into viral
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- Which subunit of the E. coli polymerase confers specificity to transcription? a. b. c. d.A botanist notices that a tomato plant looks diseased. How could the botanist confirm that the agent causing disease is a viroid, and not a virus?32. The CRISPR-Cas system helps some prokaryotes defend against the phage! Which of the following statements correctly describes how this system works? A) it produces RNA that is translated into proteins that attack viral DNA. B) it inserts specific genes randomly into the prokaryotic genome ) it causes the synthesis of proteins that are then secreted by the prokaryote that attack viruses in the environment. D) it cuts viral DNA at specific sequences before it can replicate.
- 4. Shown below is the structure of the anti-retroviral drug AZT. HOCH, H 3 N₂ HN AZT H 2 H O CH₂ a) What nucleotide does the drug resemble? b) Describe a plausible mechanism for how this drug would inhibit the growth of HIV. c) Given what you know about DNA replication, why does the drug kill the virus but not people?It was discovered that COVID19 RNA is a sense (+) strand. If the RNA sequence that codes for the 5 viral genes begins with 5’ GGGUACAUGGUAGCC …. 3’, the starting amino sequence is a) N-terminal Gly-Tyr-Met-Val-Ala- C-terminal b) N-terminal Pro-Met-Tyr-His-Arg- C-terminal c) N-terminal Val-Ala- C-terminal d) N-terminal Tyr-His-Arg- C-terminal e) N-terminal Met-Tyr-His-Arg- C-terminal8) Draw a two-stranded, antiparallel ß-sheet with at least four amino acids in each B-strand.
- 1. "One structural form used in building virus particles is based on the icosahedron. Describe, either in words or in a diagram, the organization (number of capsomers, etc.) of the simplest virus particle of this form. 2. If a virus has a negative‐sense RNA genome, what enzymatic activity (if any) will be found as part of the virion, and what will be the first step in expression of the viral genome?"27. Class VI animal viruses include HIV, Based on the type of viral genome, how is this class distinguished from the other classes of animal virus? A) It has double stranded RNA. B) It has single stranded RNA that serves as mRNA (positive sense). C) It has single stranded RNA that serves as a template for the synthesis of DNA. D) It has single stranded RNA that serves as a template for mRNA (anti-sense). does HIV1)What are the difference between RNA and DNA? 2)Describe steps of viral replication, what are the differences in the replication between RNA and DNA viruses ?
- 6. Given: 3"--TACTTCAAACCGGGCCCGATT--5 a) Give the sequence of nucleotides in the mRNA. b) What is the sequence of amino acids in the protein coded by the MRNA? c) If the first G is substituted by A, what is the sequence of amino acids in the protein? d) What type of mutation is illustrated by (e)?2. The amino acid sequences in the lysozyme protein produced by the TF phage were studied by Streisinger and co-workers. One sequence is Lys-Ser-Pro-Ser-Leu-Asn-Ala. However, the result of a single nucleotide deletion and insertion of another nucleotide, this amino acid sequence changed to Lys-Val-His-His-Leu-Met-Ala. Using the codons in Table 13.1, determine the nucleotide sequences that produced a. the original amino acid sequence; and b. the subsequent changes.C) termination 2) Given the following coding strand sequence of DNA, what is the template strand sequence? 5'-ATGCGCTGAC-3' (3) Given the following coding strand sequence of DNA, what is the corresponding mRNA sequence? 5'-ATGCGCTGAC-3' 4) Use the genetic code and translate the following DNA coding strand sequence. 5' ATG ATA AAT ACC TAG 3' 5) Introduce a mutation to the DNA sequence and describe its effect on the protein sequence.