3 or 4 or 5 1. Apeer-reviewed scientific journal articles.is usually based on either past experiences or information gained from reading2.variables are kept the same to maintain consistent testing conditions across allexperimental samples.3.provides a quick, albeit inaccurate, means of obtaining the pH of a solution.4. Substances whose pH values are above 7.00 are considered; substances whose pHvalues are below 7.00 are considered5. During areaction, molecule B receives an electron(s) from molecule A, leavingmolecule A oxidized.6. Thereagent is used to detect the presence of complex sugars (ex. starch) insolutions.7. Reducing sugars, such as glucose, will always react with Benedict's reagent because thesesugars have a(n)functional group.8.are molecules which donate protons (H+) in solution very easily.

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3. provides a quick, albeit inaccurate, means of obtaining the pH of a solution. 4. Substances whose pH values are above 7.00 are considered values are below 7.00 are considered ; substances whose pH 5. During a molecule A oxidized. reaction, molecule B receives an electron(s) from molecule A, leaving

3. provides a quick, albeit inaccurate, means of obtaining the pH of a solution. 4. Substances whose pH values are above 7.00 are considered values are below 7.00 are considered ; substances whose pH 5. During a molecule A oxidized. reaction, molecule B receives an electron(s) from molecule A, leaving

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 7P

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2. Mucic acid test – place 1 ml of the sample (lactose, galactose, glucose, fructose) in separate tubes. Add 1 ml conc. HNO3 in each tube. Heat the tubes in a boiling water bath for 1 hour. Cool at room temperature. Induce crystallization by scratching the side of the tube with a clean stirring rod, if no crystals appear; let it stand until the next laboratory period. Examine the crystals under the microscope and draw.
1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes
5. RX: Peppermint spirit 2ml Alcohol 10ml Sterile water QS 100ml Approximately how many fluid ounces of sterile water are needed to compound this solution?
10) True or False: The output of the Mass Spectrometer gives us the exact mass of a molecule.
1. Identify what test is being described: Refers to the breaking of peptide bonds that connect amino acids to compose protein ? A. Hydrolysis B. Denaturation C. Heat denaturation D. Organic solvent denaturation E. Biuret test F. Hopkins – Cole Reaction G. Millon’s test H. Ninhydrin Test I. Sulfur test J. Xanthroproteic Test K. Chromatography L. Paper chromatography M. Competitive inhibition N. Noncompetitive inhibition O. Rancidity P. Hydrogenation 2. Identify what test is being described: Test that detects the free amino group in amino acids ? A. Hydrolysis B. Denaturation C. Heat denaturation D. Organic solvent denaturation E. Biuret test F. Hopkins – Cole Reaction G. Millon’s test H. Ninhydrin Test I. Sulfur test J. Xanthroproteic Test K. Chromatography L. Paper chromatography M. Competitive inhibition N. Noncompetitive inhibition O. Rancidity P. Hydrogenation 3. Identify what test is being described: A foreign substance, which is structurally similar to the substrate, competes for…
1. Draw/illustrate the chemical reaction involved in Barfoed's test and explain the purpose of this qualitative test? 2. Draw/illustrate the chemical reaction involved in Seliwanoff's test and explain the purpose of this qualitative test? 3. Draw/illustrate the chemical reaction involved in Benedict's test and explain the purpose of this qualitative test? 4. Draw/illustrate the chemical reaction involved in Lugol's test and explain the purpose of this qualitative test?
QUESTION 3 An unknown protein was determined using the following method: Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NaOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the following test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.8ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0.1ml Unknown 0.2ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2.…
Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…
4. Create an illustrated step-by-step schematic diagram of the following Qualitative test for Lipids: Solubility Test Translucent Test Acrolein Test Baudouin Test Huble's Test
During the lumbar puncture 3 bottles are used: Why don’t they spin the sample that will be used for biochemistry?
1. Provide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". A. Biuret Test B. Xanthoproteic Test C. Millon's Test D. Ninhydrin Test E. Hopkins-Cole Test F. Sulfur Test G. Heller's Test
6. Complete the table below. If the sample is expected to show no reaction for a particular test, write NO RXN. If the sample is expected to show a reaction, describe the ideal observation for a positive result. Molisch's Bial's Test Iodine Test Benedict's test Cellulose Formation of purple interphase Formation of Fructose purple interphase Mannose Galactose